Samples with different proportions WO3/TiO2 were prepared by co-precipitation method followed by a heat treatment. The samples were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), diffuse reflectance spectroscopy (DRS), and adsorption–desorption N2 isotherms (BET). The photocatalytic properties of WO3/TiO2 samples were evaluated in the photo-oxidation reaction of nitric oxide (NO) under UV and visible light irradiation. The highest photocatalytic activity was observed in the WO3/TiO2 sample with a composition of 80% mole of TiO2. Among the different substrates used for supporting the photocatalyst, the best results were reached over concrete and glass when it was exposed to UV and visible light irradiation, respectively. In overall, the photocatalytic efficiency of the synthesized materials was higher under UV than visible light irradiation.