TY - JOUR
T1 - Surfactant-aided size exclusion chromatography
AU - Horneman, D.A.
AU - Wolbers, M.
AU - Zomerdijk, M.
AU - Ottens, M.
AU - Keurentjes, J.T.F.
AU - Wielen, van der, L.A.M.
PY - 2004
Y1 - 2004
N2 - The flexibility and selectivity of size exclusion chromatog. (SEC) for protein purifn. can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liq. phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatog. technol. (SASEC) is demonstrated on the sepn. of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper
AB - The flexibility and selectivity of size exclusion chromatog. (SEC) for protein purifn. can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liq. phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatog. technol. (SASEC) is demonstrated on the sepn. of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper
U2 - 10.1016/j.jchromb.2004.01.061
DO - 10.1016/j.jchromb.2004.01.061
M3 - Article
C2 - 15177158
SN - 1570-0232
VL - 807
SP - 39
EP - 45
JO - Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -