Surfactant-aided size exclusion chromatography

D.A. Horneman, M. Wolbers, M. Zomerdijk, M. Ottens, J.T.F. Keurentjes, L.A.M. Wielen, van der

    Research output: Contribution to journalArticleAcademicpeer-review

    10 Citations (Scopus)


    The flexibility and selectivity of size exclusion chromatog. (SEC) for protein purifn. can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liq. phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatog. technol. (SASEC) is demonstrated on the sepn. of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper
    Original languageEnglish
    Pages (from-to)39-45
    JournalJournal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences
    Issue number1
    Publication statusPublished - 2004


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