Super-resolution microscopy unveils dynamic heterogeneities in nanoparticle protein corona

Natalia Feiner-Gracia; Michaela Beck; Sílvia Pujals; Sébastien Tosi; Tamoghna Mandal; Christian Buske; Mika Linden; Lorenzo Albertazzi

doi:10.1002/smll.201701631

Super-resolution microscopy unveils dynamic heterogeneities in nanoparticle protein corona

Natalia Feiner-Gracia
, Michaela Beck
, Sílvia Pujals
, Sébastien Tosi
, Tamoghna Mandal
, Christian Buske
, Mika Linden
, Lorenzo Albertazzi

Research output: Contribution to journal › Article › Academic › peer-review

126 Citations (Scopus)

Abstract

The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle-based therapies. Here, it is shown that the use of super-resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle-by-particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody-functionalized nanoparticles providing a detailed understanding of corona-activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.

Original language	English
Article number	1701631
Number of pages	11
Journal	Small : Nano Micro
Volume	13
Issue number	41
DOIs	https://doi.org/10.1002/smll.201701631
Publication status	Published - 6 Nov 2017
Externally published	Yes

Funding

N. Feiner-Gracia, Dr. S. Pujals, Dr. L. Albertazzi Institute for Bioengineering of Catalonia (IBEC) The Barcelona Institute of Science and Technology Baldiri Reixac 15-21, 08028 Barcelona, Spain E-mail: [email protected] M. Beck, Prof. M. Linden Inorganic Chemistry II Ulm University Albert-Einstein-Allee 11, D-89081 Ulm, Germany Dr. S. Tosi Advanced Digital Microscopy Core Facility (ADMCF) Institute for Research in Biomedicine (IRB Barcelona) The Barcelona Institute of Science and Technology 08028 Barcelona, Spain T. Mandal, Prof. C. Buske Institute of Experimental Cancer Research University Hospital Ulm Albert-Einstein-Allee 11, D-89081 Ulm, Germany The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201701631. This work was financially supported by the AXA research fund (L.A.) and by the Spanish Ministry of Economy, Industry and Competitiveness (L.A., N.F.-G., and S.P.), by the Generalitat de Catalunya through the CERCA program, moreover this work was supported by the Spanish Ministry of Economy, Industry and Competitiveness through the Project SAF2016-75241-R (MINECO-FEDER). M.L. thanks Margit Lang for ICP-OES analysis. N.F-G. thanks Tania Patiño for the help with BCA test. L.A. acknowledges Daan van der Zwaag and David Caballero for useful discussions.

Keywords

heterogeneity
mesoporous silica nanoparticles
protein corona
super-resolution imaging
targeting
Silicon Dioxide/chemistry
Protein Corona/chemistry
Animals
Adsorption
Microscopy/methods
Time Factors
Cattle
Surface Properties
Nanoparticles/chemistry
Serum Albumin, Bovine/chemistry
Kinetics
Porosity

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10.1002/smll.201701631

Cite this

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title = "Super-resolution microscopy unveils dynamic heterogeneities in nanoparticle protein corona",

abstract = "The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle-based therapies. Here, it is shown that the use of super-resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle-by-particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody-functionalized nanoparticles providing a detailed understanding of corona-activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.",

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AU - Tosi, Sébastien

AU - Mandal, Tamoghna

AU - Buske, Christian

AU - Linden, Mika

AU - Albertazzi, Lorenzo

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N2 - The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle-based therapies. Here, it is shown that the use of super-resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle-by-particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody-functionalized nanoparticles providing a detailed understanding of corona-activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.

AB - The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle-based therapies. Here, it is shown that the use of super-resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle-by-particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody-functionalized nanoparticles providing a detailed understanding of corona-activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study.

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KW - targeting

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KW - Animals

KW - Adsorption

KW - Microscopy/methods

KW - Time Factors

KW - Cattle

KW - Surface Properties

KW - Nanoparticles/chemistry

KW - Serum Albumin, Bovine/chemistry

KW - Kinetics

KW - Porosity

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Super-resolution microscopy unveils dynamic heterogeneities in nanoparticle protein corona

Abstract

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Keywords

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