Study on the analogy of gel permeation chromatography and cuprophan dialysis for the isolation of uremic 'middle molecules' using model compounds

A.C. Schoots, C.A.M.G. Cramers

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Abstract

Selectives of substituted nitrobenzens in gel permeation chromatography and cuprophan membrane dialysis were compared. Glucuronide-, glucoside-, acetic acid- and lactoside-substituted p-nitrobenzens were chosen as model compounds so-called ‘middle molecules’ in uremia. It was found that there was not a single linear relationship between the substituent effects in both processes. This was because of the anomalous behaviour of the model compounds in gel permeation. A combination of adsorption and ionic rejection in the latter technique, for the various solutes, was not encountered in cuprophan membrane dialysis, which appeared to be governed only by the molecular mass or volume of the solute. Therefore, gel permeation seems to be inappropriate for the analysis or isolation of so-called middle molecules in sera of uremic patients. Dialysis or filtration on relatively inert membranes is less susceptible to anomalies.
Original languageEnglish
Pages (from-to)79-86
JournalJournal of Chromatography. Biomedical Applications
Volume497
DOIs
Publication statusPublished - 1989

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Dialysis membranes
Dialysis
Gel permeation chromatography
Permeation
Gels
Molecules
Glucuronides
Glucosides
Molecular mass
Acetic Acid
Membranes
Adsorption
uremia middle molecule toxins
cuprammonium cellulose

Cite this

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abstract = "Selectives of substituted nitrobenzens in gel permeation chromatography and cuprophan membrane dialysis were compared. Glucuronide-, glucoside-, acetic acid- and lactoside-substituted p-nitrobenzens were chosen as model compounds so-called ‘middle molecules’ in uremia. It was found that there was not a single linear relationship between the substituent effects in both processes. This was because of the anomalous behaviour of the model compounds in gel permeation. A combination of adsorption and ionic rejection in the latter technique, for the various solutes, was not encountered in cuprophan membrane dialysis, which appeared to be governed only by the molecular mass or volume of the solute. Therefore, gel permeation seems to be inappropriate for the analysis or isolation of so-called middle molecules in sera of uremic patients. Dialysis or filtration on relatively inert membranes is less susceptible to anomalies.",
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Study on the analogy of gel permeation chromatography and cuprophan dialysis for the isolation of uremic 'middle molecules' using model compounds. / Schoots, A.C.; Cramers, C.A.M.G.

In: Journal of Chromatography. Biomedical Applications, Vol. 497, 1989, p. 79-86.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Study on the analogy of gel permeation chromatography and cuprophan dialysis for the isolation of uremic 'middle molecules' using model compounds

AU - Schoots, A.C.

AU - Cramers, C.A.M.G.

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AB - Selectives of substituted nitrobenzens in gel permeation chromatography and cuprophan membrane dialysis were compared. Glucuronide-, glucoside-, acetic acid- and lactoside-substituted p-nitrobenzens were chosen as model compounds so-called ‘middle molecules’ in uremia. It was found that there was not a single linear relationship between the substituent effects in both processes. This was because of the anomalous behaviour of the model compounds in gel permeation. A combination of adsorption and ionic rejection in the latter technique, for the various solutes, was not encountered in cuprophan membrane dialysis, which appeared to be governed only by the molecular mass or volume of the solute. Therefore, gel permeation seems to be inappropriate for the analysis or isolation of so-called middle molecules in sera of uremic patients. Dialysis or filtration on relatively inert membranes is less susceptible to anomalies.

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