Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism

E.E. Beuling, S.P.P. Ottengraf, J.C. Heuvel, van den

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    To study the effect of the cell fraction f on diffusion an artificial biofilm was developed, consisting of an agar matrix contg. monodisperse impenetrable polystyrene particles with the same diam. as bacteria (1 mm). Diffusion of glucose at different polystyrene fractions was measured in two ways: with a micro-electrode the transient response on a concn. step in the well mixed bulk was recorded in time and in a classical diffusion cell the flux through a flat film was measured. Detn. of the diffusion coeff. with a micro-electrode was shown to be accurate and, therefore, a good addn. to the usual macroscopic methods. The decrease of the diffusion coeff. with increasing polystyrene fraction (0-28 % vol./vol.) was larger as expected. This might be related to an inhomogeneous distribution of the polystyrene particles in the agar and is currently under investigation. [on SciFinder (R)]
    Original languageEnglish
    Title of host publicationSecond International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK
    Place of PublicationRugby, UK
    PublisherInstitution of Chemical Engineers
    Pages125-127
    ISBN (Print)0-85295-332-1
    Publication statusPublished - 1994
    Eventconference; International Symposium on Environmental Biotechnology (ISEB) ; 2 (Brighton, UK) : 1994.07.04-06; 1994-07-04; 1994-07-06 -
    Duration: 4 Jul 19946 Jul 1994

    Conference

    Conferenceconference; International Symposium on Environmental Biotechnology (ISEB) ; 2 (Brighton, UK) : 1994.07.04-06; 1994-07-04; 1994-07-06
    Period4/07/946/07/94
    OtherInternational Symposium on Environmental Biotechnology (ISEB) ; 2 (Brighton, UK) : 1994.07.04-06

    Fingerprint

    biofilms
    microorganisms
    mass transfer
    polystyrene
    electrodes
    transient response
    cells
    glucose
    bacteria
    matrices

    Cite this

    Beuling, E. E., Ottengraf, S. P. P., & Heuvel, van den, J. C. (1994). Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism. In Second International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK (pp. 125-127). Rugby, UK: Institution of Chemical Engineers.
    Beuling, E.E. ; Ottengraf, S.P.P. ; Heuvel, van den, J.C. / Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism. Second International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK. Rugby, UK : Institution of Chemical Engineers, 1994. pp. 125-127
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    title = "Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism",
    abstract = "To study the effect of the cell fraction f on diffusion an artificial biofilm was developed, consisting of an agar matrix contg. monodisperse impenetrable polystyrene particles with the same diam. as bacteria (1 mm). Diffusion of glucose at different polystyrene fractions was measured in two ways: with a micro-electrode the transient response on a concn. step in the well mixed bulk was recorded in time and in a classical diffusion cell the flux through a flat film was measured. Detn. of the diffusion coeff. with a micro-electrode was shown to be accurate and, therefore, a good addn. to the usual macroscopic methods. The decrease of the diffusion coeff. with increasing polystyrene fraction (0-28 {\%} vol./vol.) was larger as expected. This might be related to an inhomogeneous distribution of the polystyrene particles in the agar and is currently under investigation. [on SciFinder (R)]",
    author = "E.E. Beuling and S.P.P. Ottengraf and {Heuvel, van den}, J.C.",
    year = "1994",
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    isbn = "0-85295-332-1",
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    Beuling, EE, Ottengraf, SPP & Heuvel, van den, JC 1994, Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism. in Second International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK. Institution of Chemical Engineers, Rugby, UK, pp. 125-127, conference; International Symposium on Environmental Biotechnology (ISEB) ; 2 (Brighton, UK) : 1994.07.04-06; 1994-07-04; 1994-07-06, 4/07/94.

    Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism. / Beuling, E.E.; Ottengraf, S.P.P.; Heuvel, van den, J.C.

    Second International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK. Rugby, UK : Institution of Chemical Engineers, 1994. p. 125-127.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    AB - To study the effect of the cell fraction f on diffusion an artificial biofilm was developed, consisting of an agar matrix contg. monodisperse impenetrable polystyrene particles with the same diam. as bacteria (1 mm). Diffusion of glucose at different polystyrene fractions was measured in two ways: with a micro-electrode the transient response on a concn. step in the well mixed bulk was recorded in time and in a classical diffusion cell the flux through a flat film was measured. Detn. of the diffusion coeff. with a micro-electrode was shown to be accurate and, therefore, a good addn. to the usual macroscopic methods. The decrease of the diffusion coeff. with increasing polystyrene fraction (0-28 % vol./vol.) was larger as expected. This might be related to an inhomogeneous distribution of the polystyrene particles in the agar and is currently under investigation. [on SciFinder (R)]

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    Beuling EE, Ottengraf SPP, Heuvel, van den JC. Study of mass transfer in artificial biofilm to enhance prodn. with immobilized microorganism. In Second International Symposium on Environmental Biotechnology : [ISEB '2] ; a three-day symposium, Brighton UK. Rugby, UK: Institution of Chemical Engineers. 1994. p. 125-127