Strong and reversible monovalent supramolecular protein immobilization

J.F. Young; H. Nguyen; L. Yang; J. Huskens; P. Jonkheijm; L. Brunsveld

doi:10.1002/cbic.200900599

Strong and reversible monovalent supramolecular protein immobilization

J.F. Young
, H. Nguyen
, L. Yang
, J. Huskens
, P. Jonkheijm
, L. Brunsveld

Chemical Biology

Research output: Contribution to journal › Article › Academic › peer-review

82 Citations (Scopus)

3 Downloads (Pure)

Abstract

Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.

Original language	English
Pages (from-to)	180-183
Journal	ChemBioChem
Volume	11
Issue number	2
DOIs	https://doi.org/10.1002/cbic.200900599
Publication status	Published - 2010

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title = "Strong and reversible monovalent supramolecular protein immobilization",

abstract = "Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.",

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AU - Young, J.F.

AU - Nguyen, H.

AU - Yang, L.

AU - Huskens, J.

AU - Jonkheijm, P.

AU - Brunsveld, L.

PY - 2010

Y1 - 2010

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AB - Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand.

U2 - 10.1002/cbic.200900599

DO - 10.1002/cbic.200900599

M3 - Article

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SP - 180

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JO - ChemBioChem

JF - ChemBioChem

IS - 2

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Strong and reversible monovalent supramolecular protein immobilization

Abstract

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