Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers

Y. Razskazovskiy, M.G. Debije, S.B. Howerton, L.D. Williams, W.A. Bernhard

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Direct ionization of crystalline d(CGCGCGCG) and d(CGCGCGCGCG) oligomers produces 3'- and 5'-phosphate-terminated fragments as the main strand breakage products detectable by ion-exchange chromatography. The nature of the base has no effect on the probability of strand breakage at the given site. The yields of 3'-phosphates are systematically lower than the yields of the 5'-phosphates originating from the same cleavage site, pointing to the possible presence of unidentified products with sugar remnants attached to the 3'-end. These results show that direct ionization is efficient at producing single-strand breaks in DNA and its action is relatively indiscriminate with respect to base sequence.
LanguageEnglish
Pages334-339
Number of pages6
JournalRadiation Research
Volume160
Issue number3
DOIs
StatePublished - 2003

Fingerprint

oligomers
strands
phosphates
deoxyribonucleic acid
Phosphates
DNA
Single-Stranded DNA Breaks
ionization
Ion Exchange Chromatography
chromatography
products
sugars
cleavage
fragments
ions

Cite this

Razskazovskiy, Y., Debije, M. G., Howerton, S. B., Williams, L. D., & Bernhard, W. A. (2003). Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers. Radiation Research, 160(3), 334-339. DOI: 10.1667/RR3051
Razskazovskiy, Y. ; Debije, M.G. ; Howerton, S.B. ; Williams, L.D. ; Bernhard, W.A./ Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers. In: Radiation Research. 2003 ; Vol. 160, No. 3. pp. 334-339
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Razskazovskiy, Y, Debije, MG, Howerton, SB, Williams, LD & Bernhard, WA 2003, 'Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers' Radiation Research, vol. 160, no. 3, pp. 334-339. DOI: 10.1667/RR3051

Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers. / Razskazovskiy, Y.; Debije, M.G.; Howerton, S.B.; Williams, L.D.; Bernhard, W.A.

In: Radiation Research, Vol. 160, No. 3, 2003, p. 334-339.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bernhard,W.A.

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AB - Direct ionization of crystalline d(CGCGCGCG) and d(CGCGCGCGCG) oligomers produces 3'- and 5'-phosphate-terminated fragments as the main strand breakage products detectable by ion-exchange chromatography. The nature of the base has no effect on the probability of strand breakage at the given site. The yields of 3'-phosphates are systematically lower than the yields of the 5'-phosphates originating from the same cleavage site, pointing to the possible presence of unidentified products with sugar remnants attached to the 3'-end. These results show that direct ionization is efficient at producing single-strand breaks in DNA and its action is relatively indiscriminate with respect to base sequence.

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Razskazovskiy Y, Debije MG, Howerton SB, Williams LD, Bernhard WA. Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers. Radiation Research. 2003;160(3):334-339. Available from, DOI: 10.1667/RR3051