Strand breaks in x-irradiated crystalline DNA : alternating CG-oligomers

Y. Razskazovskiy, M.G. Debije, S.B. Howerton, L.D. Williams, W.A. Bernhard

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Direct ionization of crystalline d(CGCGCGCG) and d(CGCGCGCGCG) oligomers produces 3'- and 5'-phosphate-terminated fragments as the main strand breakage products detectable by ion-exchange chromatography. The nature of the base has no effect on the probability of strand breakage at the given site. The yields of 3'-phosphates are systematically lower than the yields of the 5'-phosphates originating from the same cleavage site, pointing to the possible presence of unidentified products with sugar remnants attached to the 3'-end. These results show that direct ionization is efficient at producing single-strand breaks in DNA and its action is relatively indiscriminate with respect to base sequence.
Original languageEnglish
Pages (from-to)334-339
Number of pages6
JournalRadiation Research
Volume160
Issue number3
DOIs
Publication statusPublished - 2003

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