Strains in stratified agarose constructs as determined by displacement-encoded MRI

Adam Griebel, C.C. van Donkelaar, Corey P. Neu

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review


Osteoarthritis (OA) is a debilitating disease for which no satisfactory treatment exists. Tissue engineering-based strategies have shown considerable potential for repair. Agarose is frequently used as a scaffold material, as chondrocytes maintain their phenotype and cells remain responsive to mechanical stimuli. To improve the mechanical quality of tissue engineered cartilage, recent studies aimed to reproduce the depth-dependent structure of healthy cartilage. One approach to achieve this is by applying depth-dependent mechanical stimuli via cyclically sliding a glass cylinder over the cell-seeded agarose construct [1,2]. The different strains applied to the surface and the deeper regions are expected to induce stratified matrix synthesis and therefore stratified tissue stiffness. Consequently, with the same external stimuli, the internal strain distribution may alter with ongoing tissue development. Such effect is important to understand in order to optimize mechanical loading regimes for cartilage tissue engineering.
Original languageEnglish
Title of host publicationASME 2012 Summer Bioengineering Conference, SBC 2012
Number of pages2
Publication statusPublished - 1 Dec 2012
EventASME 2012 Summer Bioengineering Conference (SBC 2012) - Fajardo, Puerto Rico
Duration: 20 Jun 201223 Jun 2012


ConferenceASME 2012 Summer Bioengineering Conference (SBC 2012)
Abbreviated titleSBC 2012
Country/TerritoryPuerto Rico
Internet address


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