Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase

N. Taouatas, M.M. Drugan, A.J.R. Heck, Sh. Mohammed

Research output: Contribution to journalArticleAcademicpeer-review

112 Citations (Scopus)


We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.

Original languageEnglish
Pages (from-to)405-407
Number of pages3
JournalNature Methods
Issue number5
Publication statusPublished - May 2008


  • Cell Line
  • Humans
  • Lysine
  • Mass Spectrometry
  • Metalloendopeptidases
  • Peptides
  • Sequence Analysis, Protein


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