Abstract
We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.
Original language | English |
---|---|
Pages (from-to) | 405-407 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 5 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2008 |
Keywords
- Cell Line
- Humans
- Lysine
- Mass Spectrometry
- Metalloendopeptidases
- Peptides
- Sequence Analysis, Protein