Stimuli responsive peptide conjugated polymer nanoparticles

J.R.I. Knoop; M. Geus, de; G.J.M. Habraken; C.E. Koning; H. Menzel; A. Heise

doi:10.1021/ma100327p

Stimuli responsive peptide conjugated polymer nanoparticles

J.R.I. Knoop, M. Geus, de, G.J.M. Habraken, C.E. Koning, H. Menzel, A. Heise

Chemical Engineering and Chemistry

Research output: Contribution to journal › Article › Academic › peer-review

31 Citations (Scopus)

Abstract

We have investigated a novel approach to well-defined peptide-decorated cross-linked nanoparticles. The particles were obtained from well-defined poly(¿-benzyl-l-glutamate-b-styrene) block copolymers with active nitroxide end-groups by reaction with divinylbenzene (DVB). The block length ratio and the amount of cross-linker (DVB) were systematically varied. Molecular weights of the cross-linked particles up to 548000 g/mol with polydispersities around 1.5 were confirmed by size-exclusion chromatography. A clear dependence of the molecular weight from the block length ratio and the amount of cross-linker was observed. This was confirmed by dynamic light scattering results revealing the formation of nanoparticles up to 21 nm in size. The core-shell structure was evident from the TEM micrographs. Further deprotection of the peptide shell yielded water-soluble pH-responsive nanoparticles with a poly(l-glutamic acid) shell and a polystyrene core. Cryo-TEM micrographs confirmed the presence of individual core-shell particles in the aqueous solution.

Original language	English
Pages (from-to)	4126-4132
Journal	Macromolecules
Volume	43
Issue number	9
DOIs	https://doi.org/10.1021/ma100327p
Publication status	Published - 2010

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title = "Stimuli responsive peptide conjugated polymer nanoparticles",

abstract = "We have investigated a novel approach to well-defined peptide-decorated cross-linked nanoparticles. The particles were obtained from well-defined poly(¿-benzyl-l-glutamate-b-styrene) block copolymers with active nitroxide end-groups by reaction with divinylbenzene (DVB). The block length ratio and the amount of cross-linker (DVB) were systematically varied. Molecular weights of the cross-linked particles up to 548000 g/mol with polydispersities around 1.5 were confirmed by size-exclusion chromatography. A clear dependence of the molecular weight from the block length ratio and the amount of cross-linker was observed. This was confirmed by dynamic light scattering results revealing the formation of nanoparticles up to 21 nm in size. The core-shell structure was evident from the TEM micrographs. Further deprotection of the peptide shell yielded water-soluble pH-responsive nanoparticles with a poly(l-glutamic acid) shell and a polystyrene core. Cryo-TEM micrographs confirmed the presence of individual core-shell particles in the aqueous solution.",

author = "J.R.I. Knoop and {Geus, de}, M. and G.J.M. Habraken and C.E. Koning and H. Menzel and A. Heise",

year = "2010",

doi = "10.1021/ma100327p",

language = "English",

volume = "43",

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journal = "Macromolecules",

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TY - JOUR

T1 - Stimuli responsive peptide conjugated polymer nanoparticles

AU - Knoop, J.R.I.

AU - Geus, de, M.

AU - Habraken, G.J.M.

AU - Koning, C.E.

AU - Menzel, H.

AU - Heise, A.

PY - 2010

Y1 - 2010

N2 - We have investigated a novel approach to well-defined peptide-decorated cross-linked nanoparticles. The particles were obtained from well-defined poly(¿-benzyl-l-glutamate-b-styrene) block copolymers with active nitroxide end-groups by reaction with divinylbenzene (DVB). The block length ratio and the amount of cross-linker (DVB) were systematically varied. Molecular weights of the cross-linked particles up to 548000 g/mol with polydispersities around 1.5 were confirmed by size-exclusion chromatography. A clear dependence of the molecular weight from the block length ratio and the amount of cross-linker was observed. This was confirmed by dynamic light scattering results revealing the formation of nanoparticles up to 21 nm in size. The core-shell structure was evident from the TEM micrographs. Further deprotection of the peptide shell yielded water-soluble pH-responsive nanoparticles with a poly(l-glutamic acid) shell and a polystyrene core. Cryo-TEM micrographs confirmed the presence of individual core-shell particles in the aqueous solution.

AB - We have investigated a novel approach to well-defined peptide-decorated cross-linked nanoparticles. The particles were obtained from well-defined poly(¿-benzyl-l-glutamate-b-styrene) block copolymers with active nitroxide end-groups by reaction with divinylbenzene (DVB). The block length ratio and the amount of cross-linker (DVB) were systematically varied. Molecular weights of the cross-linked particles up to 548000 g/mol with polydispersities around 1.5 were confirmed by size-exclusion chromatography. A clear dependence of the molecular weight from the block length ratio and the amount of cross-linker was observed. This was confirmed by dynamic light scattering results revealing the formation of nanoparticles up to 21 nm in size. The core-shell structure was evident from the TEM micrographs. Further deprotection of the peptide shell yielded water-soluble pH-responsive nanoparticles with a poly(l-glutamic acid) shell and a polystyrene core. Cryo-TEM micrographs confirmed the presence of individual core-shell particles in the aqueous solution.

U2 - 10.1021/ma100327p

DO - 10.1021/ma100327p

M3 - Article

SN - 0024-9297

VL - 43

SP - 4126

EP - 4132

JO - Macromolecules

JF - Macromolecules

IS - 9

ER -

Stimuli responsive peptide conjugated polymer nanoparticles

Abstract

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