Proton microprobe techniques are useful in investigating ischemia-reperfusion induced ion shifts between cardiac muscle cells and interstitial fluid. Preliminary results have shown that proper analysis of ion concentrations in various cardiac compartments is hampered by dislocation of elements during sample preparation. In this study the different stages of the preparation were evaluated as possible sources of artifacts. After improvements of the sample preparation procedure, sharp ion concentration gradients within heart tissue preparations were obtained, indicating no noticeable ion dislocation at the scale of the measurements.
|Number of pages||5|
|Journal||Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms|
|Publication status||Published - 1999|
Quaedackers, J. A., Mutsaers, P. H. A., de Goeij, J. J. M., Voigt, de, M. J. A., & Vusse, van der, G. J. (1999). State and history of heart tissue preparation for proton microprobe elemental analysis at the Eindhoven Cyclotron Laboratory. Nuclear Instruments and Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms, 158(1-4), 399-404. https://doi.org/10.1016/S0168-583X(99)00502-9