Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

Arianna Mauretti; Fabrizio Rossi; Noortje A.M. Bax; Carmen Miano; Fabio Miraldi; Marie José Goumans; Elisa Messina; Alessandro Giacomello; Carlijn V.C. Bouten; Cecilia Sahlgren

doi:10.1557/mrc.2017.82

Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

Arianna Mauretti, Fabrizio Rossi, Noortje A.M. Bax, Carmen Miano, Fabio Miraldi, Marie José Goumans, Elisa Messina, Alessandro Giacomello, Carlijn V.C. Bouten, Cecilia Sahlgren

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Abstract

Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O₂ further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

Original language	English
Pages (from-to)	496-501
Number of pages	6
Journal	MRS Communications
Volume	7
Issue number	3
DOIs	https://doi.org/10.1557/mrc.2017.82
Publication status	Published - 1 Sept 2017

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AU - Mauretti, Arianna

AU - Rossi, Fabrizio

AU - Bax, Noortje A.M.

AU - Miano, Carmen

AU - Miraldi, Fabio

AU - Goumans, Marie José

AU - Messina, Elisa

AU - Giacomello, Alessandro

AU - Bouten, Carlijn V.C.

AU - Sahlgren, Cecilia

PY - 2017/9/1

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AB - Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

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Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

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