SPECT imaging of fibrin using fibrin-binding peptides

L.W.E. Starmans, S.M.J. Duijnhoven, van, R. Rossin, S. Aime, M.J.A.P. Daemen, K. Nicolay, H. Grüll

    Research output: Contribution to journalArticleAcademicpeer-review

    18 Citations (Scopus)
    68 Downloads (Pure)

    Abstract

    Noninvasive detection of fibrin in vivo using diagnostic imaging modalities may improve clinical decision-making on possible therapeutic options in atherosclerosis, cancer and thrombus-related pathologies such as pulmonary embolism and deep venous thrombosis. The aim of this study was to assess the potential of a novel 111In-labeled fibrin-binding peptide (FibPep) to visualize thrombi in mice noninvasively using single-photon emission computed tomography (SPECT). FibPep and a negative control peptide (NCFibPep) were synthesized and their fibrin-binding properties were assessed in vitro. FibPep showed enhanced binding compared with NCFibPep to both fibrin and blood clots. FibPep bound to fibrin with a dissociation constant (Kd) of 0.8 m, whereas NCFibPep displayed at least a 100-fold lower affinity towards fibrin. A FeCl3-injury carotid artery thrombosis mouse model was used to evaluate the peptides in vivo. FibPep and NCFibPep displayed rapid blood clearance and were eliminated via the renal pathway. In vivo SPECT imaging using FibPep allowed clear visualization of thrombi. Ex vivo biodistribution showed significantly increased uptake of FibPep in the thrombus-containing carotid in comparison to the noninjured carotid (5.7 +/- 0.7 and 0.6 +/- 0.4% injected dose per gram (%ID g1), respectively; p
    Original languageEnglish
    Pages (from-to)229-237
    Number of pages9
    JournalContrast Media and Molecular Imaging
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Dive into the research topics of 'SPECT imaging of fibrin using fibrin-binding peptides'. Together they form a unique fingerprint.

    Cite this