Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Matt Timmers; Wouter Peeters; Niels J Hauwert; Cristianne J F Rijcken; Tina Vermonden; Ingrid Dijkgraaf; Rob M J Liskamp

doi:10.1039/d3cc03397j

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Matt Timmers (Corresponding author), Wouter Peeters, Niels J Hauwert, Cristianne J F Rijcken, Tina Vermonden, Ingrid Dijkgraaf (Corresponding author), Rob M J Liskamp (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

Original language	English
Pages (from-to)	11397-11400
Number of pages	4
Journal	Chemical Communications, ChemComm
Volume	59
Issue number	76
DOIs	https://doi.org/10.1039/d3cc03397j
Publication status	Published - 28 Sept 2023
Externally published	Yes

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title = "Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition",

abstract = "The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc. ",

author = "Matt Timmers and Wouter Peeters and Hauwert, {Niels J} and Rijcken, {Cristianne J F} and Tina Vermonden and Ingrid Dijkgraaf and Liskamp, {Rob M J}",

year = "2023",

month = sep,

day = "28",

doi = "10.1039/d3cc03397j",

language = "English",

volume = "59",

pages = "11397--11400",

journal = "Chemical Communications, ChemComm",

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publisher = "Royal Society of Chemistry",

number = "76",

}

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition. / Timmers, Matt (Corresponding author); Peeters, Wouter; Hauwert, Niels J et al.
In: Chemical Communications, ChemComm, Vol. 59, No. 76, 28.09.2023, p. 11397-11400.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

AU - Timmers, Matt

AU - Peeters, Wouter

AU - Hauwert, Niels J

AU - Rijcken, Cristianne J F

AU - Vermonden, Tina

AU - Dijkgraaf, Ingrid

AU - Liskamp, Rob M J

PY - 2023/9/28

Y1 - 2023/9/28

N2 - The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

AB - The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

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U2 - 10.1039/d3cc03397j

DO - 10.1039/d3cc03397j

M3 - Article

C2 - 37668179

SN - 1359-7345

VL - 59

SP - 11397

EP - 11400

JO - Chemical Communications, ChemComm

JF - Chemical Communications, ChemComm

IS - 76

ER -

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

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