Soft PEG-hydrogels with independently tunable stiffness and RGDS-content for cell adhesion studies

A.M. Jonker; S.A. Bode; A.H. Kusters; J.C.M. van Hest; D.W.P.M. Löwik

doi:10.1002/mabi.201500110

Soft PEG-hydrogels with independently tunable stiffness and RGDS-content for cell adhesion studies

A.M. Jonker, S.A. Bode, A.H. Kusters, J.C.M. van Hest, D.W.P.M. Löwik

Research output: Contribution to journal › Article › Academic › peer-review

30 Citations (Scopus)

Abstract

Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by cross-linking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer density and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL^-1) and the amount of RGDS moieties (0-100%) independently of each other. Rheology studies confirmed the soft nature of the hydrogels (G′ = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL^-1 gels, but with increased hydrogel stiffness (30 mg · mL^-1), their cellular adhesion decreased significantly.

Original language	English
Pages (from-to)	1338-1347
Number of pages	10
Journal	Macromolecular Bioscience
Volume	15
Issue number	10
DOIs	https://doi.org/10.1002/mabi.201500110
Publication status	Published - 1 Oct 2015
Externally published	Yes

Keywords

cell adhesion
hydrogel
SPAAC
stiffness

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abstract = "Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by cross-linking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer density and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL-1) and the amount of RGDS moieties (0-100%) independently of each other. Rheology studies confirmed the soft nature of the hydrogels (G′ = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL-1 gels, but with increased hydrogel stiffness (30 mg · mL-1), their cellular adhesion decreased significantly.",

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AU - Jonker, A.M.

AU - Bode, S.A.

AU - Kusters, A.H.

AU - van Hest, J.C.M.

AU - Löwik, D.W.P.M.

PY - 2015/10/1

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N2 - Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by cross-linking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer density and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL-1) and the amount of RGDS moieties (0-100%) independently of each other. Rheology studies confirmed the soft nature of the hydrogels (G′ = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL-1 gels, but with increased hydrogel stiffness (30 mg · mL-1), their cellular adhesion decreased significantly.

AB - Poly(ethylene)glycol (PEG)-based hydrogels are often used as matrix material for cell culturing. An efficient method to prepare soft PEG gels is by cross-linking via copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). Here, the effect of polymer density and RGDS-content on hydrogel formation and cell adhesion was studied, by varying the total polymer content (10, 20 and 30 mg · mL-1) and the amount of RGDS moieties (0-100%) independently of each other. Rheology studies confirmed the soft nature of the hydrogels (G′ = 25-2 298 Pa). HOS cells are able to adhere well to all RGDS-containing gels. Interestingly, both HeLa cells and NIH 3T3 fibroblasts showed substantial adherence to 10 and 20 mg · mL-1 gels, but with increased hydrogel stiffness (30 mg · mL-1), their cellular adhesion decreased significantly.

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Soft PEG-hydrogels with independently tunable stiffness and RGDS-content for cell adhesion studies

Abstract

Keywords

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