Site-specific peptide and protein immobilization on surface plasmon resonance chips via strain-promoted cycloaddition

A.E.M. Wammes, M.J.E. Fischer, N.J. De Mol, M.B. van Eldijk, F.P.J.T. Rutjes, J.C.M. Hest, van, F.L. van Delft

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) is a powerful label-free diagnostic tool to study biomolecular interactions. However, one of the drawbacks of SPR is the lack of controlled immobilization of ligands on the sensor surface. We have developed a modular platform for the fast, reagent-free and site-specific immobilization of azide-containing ligands by strain-promoted cycloaddition onto a cyclooctyne-modified SPR sensor surface. The usefulness of the concept was shown in a study with a papain model system, and up to 150 experiments were performed without loss of surface quality. Furthermore, azide-containing green fluorescent protein (GFP) was also effectively immobilized. Taken together, cyclooctyne-modified SPR chips enable smooth and site-selective immobilization of ligands and prove to be more robust than traditionally functionalized systems.

Original languageEnglish
Pages (from-to)1863-1867
Number of pages5
JournalLab on a Chip
Volume13
Issue number10
DOIs
Publication statusPublished - 2013
Externally publishedYes

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