Site-specific immobilization of DNA in glass microchannels via photolithography

T. Vong, J. ter Maat, T.A. van Beek, B. van Lagen, M. Giesbers, J.C.M. van Hest, H. Zuilhof

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

For the first time, a microchannel was photochemically patterned with a functional linker. This simple method was developed for the site-specific attachment of DNA via this linker onto silicon oxide surfaces (e.g., fused silica and borosilicate glass), both onto a flat surface and onto the inside of a fused silica microchannel. Sharp boundaries in the micrometer range between modified and unmodified zones were demonstrated by the attachment of fluorescently labeled DNA oligomers. Studies of repeated hybridization- dehybridization cycles revealed selective and reversible binding of cDNA strands at the explicit locations. On average, ∼7x 1011 fluorescently labeled DNA molecules were hybridized per square centimeter. The modified surfaces were characterized with X-ray photoelectron spectroscopy, infrared microscopy, static contact angle measurements, confocal laser scanning microscopy, and fluorescence detection (to quantify the attachment of the fluorescently labeled DNA).

Original languageEnglish
Pages (from-to)13952-13958
Number of pages7
JournalLangmuir
Volume25
Issue number24
DOIs
Publication statusPublished - 15 Dec 2009
Externally publishedYes

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