Single-Molecule Optical Biosensing: Recent Advances and Future Challenges

Swayandipta Dey; Mathias Dolci; Peter Zijlstra

doi:10.1021/acsphyschemau.2c00061

Single-Molecule Optical Biosensing: Recent Advances and Future Challenges

Swayandipta Dey
, Mathias Dolci
, Peter Zijlstra (Corresponding author)

Research output: Contribution to journal › Review article › peer-review

76 Citations (Scopus)

323 Downloads (Pure)

Abstract

In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems. As a result, single-molecule sensitivity has been reported in a range of biosensing assay formats. In this Perspective, we summarize optical sensors that achieve single-molecule sensitivity in direct label-free assays, sandwich assays, and competitive assays. We describe the advantages and disadvantages of single-molecule assays and summarize future challenges in the field including their optical miniaturization and integration, multimodal sensing capabilities, accessible time scales, and compatibility with real-life matrices such as biological fluids. We conclude by highlighting the possible application areas of optical single-molecule sensors that include not only healthcare but also the monitoring of the environment and industrial processes.

Original language	English
Pages (from-to)	143-156
Number of pages	14
Journal	ACS Physical Chemistry Au
Volume	3
Issue number	2
Early online date	12 Jan 2023
DOIs	https://doi.org/10.1021/acsphyschemau.2c00061
Publication status	Published - 22 Mar 2023

Bibliographical note

Funding Information:
This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 864772). This publication is part of the project “scalable lab-on-fiber optical sensing” (with project number 18477) which is financed by the Dutch Research Council (NWO).

Funding

This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 864772). This publication is part of the project “scalable lab-on-fiber optical sensing” (with project number 18477) which is financed by the Dutch Research Council (NWO).

Keywords

binding kinetics
continuous monitoring
fluorescence
multimodal sensors
nanoparticles
Single-molecule sensing

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title = "Single-Molecule Optical Biosensing: Recent Advances and Future Challenges",

abstract = "In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems. As a result, single-molecule sensitivity has been reported in a range of biosensing assay formats. In this Perspective, we summarize optical sensors that achieve single-molecule sensitivity in direct label-free assays, sandwich assays, and competitive assays. We describe the advantages and disadvantages of single-molecule assays and summarize future challenges in the field including their optical miniaturization and integration, multimodal sensing capabilities, accessible time scales, and compatibility with real-life matrices such as biological fluids. We conclude by highlighting the possible application areas of optical single-molecule sensors that include not only healthcare but also the monitoring of the environment and industrial processes.",

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AU - Zijlstra, Peter

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AB - In recent years, the sensitivity and specificity of optical sensors has improved tremendously due to improvements in biochemical functionalization protocols and optical detection systems. As a result, single-molecule sensitivity has been reported in a range of biosensing assay formats. In this Perspective, we summarize optical sensors that achieve single-molecule sensitivity in direct label-free assays, sandwich assays, and competitive assays. We describe the advantages and disadvantages of single-molecule assays and summarize future challenges in the field including their optical miniaturization and integration, multimodal sensing capabilities, accessible time scales, and compatibility with real-life matrices such as biological fluids. We conclude by highlighting the possible application areas of optical single-molecule sensors that include not only healthcare but also the monitoring of the environment and industrial processes.

KW - binding kinetics

KW - continuous monitoring

KW - fluorescence

KW - multimodal sensors

KW - nanoparticles

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Single-Molecule Optical Biosensing: Recent Advances and Future Challenges

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Keywords

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