Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

LanguageEnglish
Pages1-7
Number of pages7
JournalMicroelectronic Engineering
Volume197
DOIs
StatePublished - 5 Oct 2018

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Syringes
Micromachining
replicas
Molding
Adhesives
pumping
trapping
Cells
Pumps
Fabrication
Lasers
cells
syringes
laser machining
micromachining
adhesives
pumps
fabrication
products

Keywords

  • Brain-on-a-chip
  • NOA81
  • Passive pumping
  • Replica moulding
  • Single cell analysis

Cite this

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title = "Single cell trapping by capillary pumping using NOA81 replica moulded stencils",
abstract = "In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80{\%} with high cell survival rates (90{\%} over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.",
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author = "Emma Moonen and Regina Luttge and Frimat, {Jean Philippe}",
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Single cell trapping by capillary pumping using NOA81 replica moulded stencils. / Moonen, Emma; Luttge, Regina; Frimat, Jean Philippe.

In: Microelectronic Engineering, Vol. 197, 05.10.2018, p. 1-7.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Single cell trapping by capillary pumping using NOA81 replica moulded stencils

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AU - Luttge,Regina

AU - Frimat,Jean Philippe

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AB - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

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