Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Emma Moonen; Regina Luttge; Jean Philippe Frimat

doi:10.1016/j.mee.2018.04.010

Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Emma Moonen, Regina Luttge, Jean Philippe Frimat

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

195 Downloads (Pure)

Abstract

In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Microelectronic Engineering
Volume	197
DOIs	https://doi.org/10.1016/j.mee.2018.04.010
Publication status	Published - 5 Oct 2018

Funding

This research is financially supported by the ERC grant 280281 (MESOTAS) and the ERC-PoC MESOTAS SIEVE grant 713732 . The authors would also like to acknowledge Fer Radstake for his contribution with the development of the calcium imaging software.

Keywords

Brain-on-a-chip
NOA81
Passive pumping
Replica moulding
Single cell analysis

Access to Document

10.1016/j.mee.2018.04.010

Publishers VersionFinal published version, 2.5 MBLicence: CC BY-NC-ND

Cite this

@article{51d157c252bf4b9ea19d00b14b596d38,

title = "Single cell trapping by capillary pumping using NOA81 replica moulded stencils",

abstract = "In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.",

keywords = "Brain-on-a-chip, NOA81, Passive pumping, Replica moulding, Single cell analysis",

author = "Emma Moonen and Regina Luttge and Frimat, {Jean Philippe}",

year = "2018",

month = oct,

day = "5",

doi = "10.1016/j.mee.2018.04.010",

language = "English",

volume = "197",

pages = "1--7",

journal = "Microelectronic Engineering",

issn = "0167-9317",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Single cell trapping by capillary pumping using NOA81 replica moulded stencils

AU - Moonen, Emma

AU - Luttge, Regina

AU - Frimat, Jean Philippe

PY - 2018/10/5

Y1 - 2018/10/5

N2 - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

AB - In this contribution, we demonstrate that the optical adhesive NOA81 (Norland Products Inc.) can be used to replicate optically transparent single cell microsieve structures with exquisite resolution, enabling the fabrication of cheap stencils for single cell trapping applications by the combination of replica moulding and laser micromachining. In addition, we demonstrate an interesting capillary pumping mechanism for gently loading single neuronal cells which eliminates the need for equipment such as pumps and syringes. We demonstrate that capillary pumping through a microsieve generates gentle cell trapping velocities (<13.3 μm/s), enabling reproducible cell trapping efficiencies of 80% with high cell survival rates (90% over 1 week of culture) and facilitating the formation of spatially standardized neuronal networks.

KW - Brain-on-a-chip

KW - NOA81

KW - Passive pumping

KW - Replica moulding

KW - Single cell analysis

UR - http://www.scopus.com/inward/record.url?scp=85047100300&partnerID=8YFLogxK

U2 - 10.1016/j.mee.2018.04.010

DO - 10.1016/j.mee.2018.04.010

M3 - Article

AN - SCOPUS:85047100300

SN - 0167-9317

VL - 197

SP - 1

EP - 7

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Single cell trapping by capillary pumping using NOA81 replica moulded stencils

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this