Abstract
Monocytes continuously adapt their shapes for proper circulation and elicitation of effective immune responses. Although these functions depend on the cell mechanical properties, the mechanical behavior of monocytes is still poorly understood and accurate physiologically relevant data on basic mechanical properties are lacking almost entirely. By combining several complementary single-cell force spectroscopy techniques, we report that the mechanical properties of human monocyte are strain-rate dependent, and that chemokines can induce alterations in viscoelastic behavior. In addition, our findings indicate that human monocytes are heterogeneous mechanically and this heterogeneity is regulated by chemokine CCL2. The technology presented here can be readily used to reveal mechanical complexity of the blood cell population in disease conditions, where viscoelastic properties may serve as physical biomarkers for disease progression and response to therapy.
| Original language | English |
|---|---|
| Article number | 103555 |
| Number of pages | 13 |
| Journal | iScience |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 21 Jan 2022 |
Bibliographical note
Funding Information:AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. Conceptualization: AM; Methodology: TMJE, VS, MCH, CS, THMO, AM; Investigation: TMJE, VS, CS, AM; Visualization: TMJE, VS; Funding acquisition: AM; Project administration: AM; Supervision: AM; Writing ? original draft: TMJE, VS, AM; Writing ? review & editing: TMJE, VS, MCH, CS, THMO, AM. The authors declare no competing interests.
Funding
AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. AM and TMJE acknowledge the support by the Netherlands Organization for Scientific Research (NWO-TTW, grant number 16249). We thank Barbara Scalvini (Leiden University) for assistance in data analysis and statistics. We also thank Mehrad Babaei (Leiden University), Anatoly Golovnev (Leiden University) and Valentin Romanov (Victor Chang Cardiac Research Institute) for helpful discussions. Conceptualization: AM; Methodology: TMJE, VS, MCH, CS, THMO, AM; Investigation: TMJE, VS, CS, AM; Visualization: TMJE, VS; Funding acquisition: AM; Project administration: AM; Supervision: AM; Writing ? original draft: TMJE, VS, AM; Writing ? review & editing: TMJE, VS, MCH, CS, THMO, AM. The authors declare no competing interests.
Keywords
- Biomechanics
- Cell biology
- Immunology
