Simultaneous intracellular chloride and pH measurements using a GFP-based sensor

Daniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi, Fabio Beltram

Research output: Contribution to journalArticleAcademicpeer-review

133 Citations (Scopus)

Abstract

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.

Original languageEnglish
Pages (from-to)516-518
Number of pages3
JournalNature Methods
Volume7
Issue number7
DOIs
Publication statusPublished - 1 Jul 2010
Externally publishedYes

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