Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study

X.W. Lou; Q. Zhu; Z. Lei; J.L.J. Dongen, van; E.W. Meijer

doi:10.1016/j.chroma.2003.12.023

Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study

X.W. Lou, Q. Zhu, Z. Lei, J.L.J. Dongen, van, E.W. Meijer

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Abstract

A simple chromatog. model was used to simulate the retention behavior of supramol. complex in size exclusion chromatog. (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to study the influence of various parameters in the SEC characterization/confirmation of supramol. assocn. Peak tailing, peak fronting, peak splitting and retention time shifting were obsd. under different conditions. The chromatog. peak shape and retention time were strongly affected by the assocn. const., sample concn., as well as the addn. of a reactant in the mobile phase. Also, using the same model, the chromatog. process of Hummel-Dreyer procedure was simulated, and the procedure can be a good method for the detn. of assocn. consts. for AB type complexes

Original language	English
Pages (from-to)	67-75
Number of pages	9
Journal	Journal of Chromatography, A
Volume	1029
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2003.12.023
Publication status	Published - 2004

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title = "Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study",

abstract = "A simple chromatog. model was used to simulate the retention behavior of supramol. complex in size exclusion chromatog. (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to study the influence of various parameters in the SEC characterization/confirmation of supramol. assocn. Peak tailing, peak fronting, peak splitting and retention time shifting were obsd. under different conditions. The chromatog. peak shape and retention time were strongly affected by the assocn. const., sample concn., as well as the addn. of a reactant in the mobile phase. Also, using the same model, the chromatog. process of Hummel-Dreyer procedure was simulated, and the procedure can be a good method for the detn. of assocn. consts. for AB type complexes",

author = "X.W. Lou and Q. Zhu and Z. Lei and {Dongen, van}, J.L.J. and E.W. Meijer",

year = "2004",

doi = "10.1016/j.chroma.2003.12.023",

language = "English",

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journal = "Journal of Chromatography, A",

issn = "0021-9673",

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T1 - Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study

AU - Lou, X.W.

AU - Zhu, Q.

AU - Lei, Z.

AU - Dongen, van, J.L.J.

AU - Meijer, E.W.

PY - 2004

Y1 - 2004

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AB - A simple chromatog. model was used to simulate the retention behavior of supramol. complex in size exclusion chromatog. (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to study the influence of various parameters in the SEC characterization/confirmation of supramol. assocn. Peak tailing, peak fronting, peak splitting and retention time shifting were obsd. under different conditions. The chromatog. peak shape and retention time were strongly affected by the assocn. const., sample concn., as well as the addn. of a reactant in the mobile phase. Also, using the same model, the chromatog. process of Hummel-Dreyer procedure was simulated, and the procedure can be a good method for the detn. of assocn. consts. for AB type complexes

U2 - 10.1016/j.chroma.2003.12.023

DO - 10.1016/j.chroma.2003.12.023

M3 - Article

VL - 1029

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JO - Journal of Chromatography, A

JF - Journal of Chromatography, A

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