Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study

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Abstract

A simple chromatog. model was used to simulate the retention behavior of supramol. complex in size exclusion chromatog. (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to study the influence of various parameters in the SEC characterization/confirmation of supramol. assocn. Peak tailing, peak fronting, peak splitting and retention time shifting were obsd. under different conditions. The chromatog. peak shape and retention time were strongly affected by the assocn. const., sample concn., as well as the addn. of a reactant in the mobile phase. Also, using the same model, the chromatog. process of Hummel-Dreyer procedure was simulated, and the procedure can be a good method for the detn. of assocn. consts. for AB type complexes
Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalJournal of Chromatography, A
Volume1029
Issue number1-2
DOIs
Publication statusPublished - 2004

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