The use of a spinning lens test to determine ex vivo the shear modulus of 22 isolated human lens nuclei with ages ranging from 34 to 63 years is described. In this test procedure, the lens nucleus is spun about its polar axis. Images of the nucleus viewed from directions perpendicular to the polar axis are collected; these are used to quantify the deformations induced in the nucleus by the rotational motion. Data on these deformations are used to infer, by applying finite element inverse analysis, values for the shear modulus of the nucleus. The data on shear modulus obtained from this test program indicate that the nucleus stiffens very rapidly with age. These data are shown to compare well with the results of a related study (Wilde et al., 2012) in which the shear modulus of the nucleus is determined by similar spinning lens tests conducted on the entire lens substance.