Set-up and screening of a fragment library targeting the 14-3-3 protein interface

Dario Valenti, João Filipe Neves, François Xavier Cantrelle, Stanimira Hristeva, Domenico Lentini Santo, Tomáš Obšil, Xavier Hanoulle, Laura M. Levy, Dimitrios Tzalis, Isabelle Landrieu (Corresponding author), Christian Ottmann (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Protein-protein interactions (PPIs) are at the core of regulation mechanisms in biological systems and consequently became an attractive target for therapeutic intervention. PPIs involving the adapter protein 14-3-3 are representative examples given the broad range of partner proteins forming a complex with one of its seven human isoforms. Given the challenges represented by the nature of these interactions, fragment-based approaches offer a valid alternative for the development of PPI modulators. After having assembled a fragment set tailored on PPIs' modulation, we started a screening campaign on the sigma isoform of 14-3-3 adapter proteins. Through the use of both mono-and bi-dimensional nuclear magnetic resonance spectroscopy measurements, coupled with differential scanning fluorimetry, three fragment hits were identified. These molecules bind the protein at two different regions distant from the usual binding groove highlighting new possibilities for selective modulation of 14-3-3 complexes.

Original languageEnglish
Pages (from-to)1796-1802
Number of pages7
JournalMedChemComm
Volume10
Issue number10
DOIs
Publication statusPublished - 22 Jul 2019

Fingerprint

Dive into the research topics of 'Set-up and screening of a fragment library targeting the 14-3-3 protein interface'. Together they form a unique fingerprint.

Cite this