Separation of nucleotides by isotachophoresis

J.L. Beckers, F.M. Everaerts

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

The mono-, di-, and triphosphates of adenosine, cytidine, guanosine, and uridine were sepd. by isotachophoresis in several different electrolyte systems. At lower pH, the differences in step-height were greater, and complete sepn. of a mixt. of UMP, UTP, UDP, GDP, GMP, AMP, ADP, and CMP was obtained in 30 min at pH 3.7 with 0.01N HCl and 1-naphthylamine as electrolyte and caproic acid as terminator. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)380-385
JournalJournal of Chromatography
Volume71
Issue number2
DOIs
Publication statusPublished - 1972

Fingerprint

Isotachophoresis
Adenosine Diphosphate
Electrolytes
Nucleotides
1-Naphthylamine
Uridine Monophosphate
Cytidine Monophosphate
Cytidine
Uridine Triphosphate
Uridine Diphosphate
Guanosine
Diphosphates
Uridine
Adenosine Monophosphate
Adenosine Triphosphate

Cite this

Beckers, J.L. ; Everaerts, F.M. / Separation of nucleotides by isotachophoresis. In: Journal of Chromatography. 1972 ; Vol. 71, No. 2. pp. 380-385.
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Separation of nucleotides by isotachophoresis. / Beckers, J.L.; Everaerts, F.M.

In: Journal of Chromatography, Vol. 71, No. 2, 1972, p. 380-385.

Research output: Contribution to journalArticleAcademicpeer-review

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AB - The mono-, di-, and triphosphates of adenosine, cytidine, guanosine, and uridine were sepd. by isotachophoresis in several different electrolyte systems. At lower pH, the differences in step-height were greater, and complete sepn. of a mixt. of UMP, UTP, UDP, GDP, GMP, AMP, ADP, and CMP was obtained in 30 min at pH 3.7 with 0.01N HCl and 1-naphthylamine as electrolyte and caproic acid as terminator. [on SciFinder (R)]

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