Separation-encoded microparticles for single-cell western blotting

Burcu Gumuscu, Amy E. Herr

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Direct measurement of proteins from single cells has been realized at the microscale using microfluidic channels, capillaries, and semi-enclosed microwell arrays. Although powerful, these formats are constrained, with the enclosed geometries proving cumbersome for multistage assays, including electrophoresis followed by immunoprobing. We introduce a hybrid microfluidic format that toggles between a planar microwell array and a suspension of microparticles. The planar array is stippled in a thin sheet of polyacrylamide gel, for efficient single-cell isolation and protein electrophoresis of hundreds-to-thousands of cells. Upon mechanical release, array elements become a suspension of separation-encoded microparticles for more efficient immunoprobing due to enhanced mass transfer. Dehydrating microparticles offer improved analytical sensitivity owing to in-gel concentration of fluorescence signal for high-throughput single-cell targeted proteomics.
Original languageEnglish
Pages (from-to)64-73
Number of pages10
JournalLab on a Chip
Volume20
Issue number1
DOIs
Publication statusPublished - 7 Jan 2020
Externally publishedYes

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