Selective chemical imaging of static actin in live cells

Lech Gustav Milroy; Stefano Rizzo; Abram Calderon; Bernhard Ellinger; Silke Erdmann; Justine Mondry; Peter Verveer; Philippe Bastiaens; Herbert Waldmann; Leif Dehmelt; Hans Dieter Arndt

doi:10.1021/ja211708z

Selective chemical imaging of static actin in live cells

Lech Gustav Milroy, Stefano Rizzo, Abram Calderon, Bernhard Ellinger, Silke Erdmann, Justine Mondry, Peter Verveer, Philippe Bastiaens, Herbert Waldmann, Leif Dehmelt, Hans Dieter Arndt

Chemical Biology

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

Original language	English
Pages (from-to)	8480-8486
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	134
Issue number	20
DOIs	https://doi.org/10.1021/ja211708z
Publication status	Published - 23 May 2012

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AU - Milroy, Lech Gustav

AU - Rizzo, Stefano

AU - Calderon, Abram

AU - Ellinger, Bernhard

AU - Erdmann, Silke

AU - Mondry, Justine

AU - Verveer, Peter

AU - Bastiaens, Philippe

AU - Waldmann, Herbert

AU - Dehmelt, Leif

AU - Arndt, Hans Dieter

PY - 2012/5/23

Y1 - 2012/5/23

N2 - We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

AB - We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

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JO - Journal of the American Chemical Society

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Selective chemical imaging of static actin in live cells

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