Selective chemical imaging of static actin in live cells

Lech Gustav Milroy, Stefano Rizzo, Abram Calderon, Bernhard Ellinger, Silke Erdmann, Justine Mondry, Peter Verveer, Philippe Bastiaens, Herbert Waldmann, Leif Dehmelt, Hans Dieter Arndt

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40 Citations (Scopus)

Abstract

We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.

Original languageEnglish
Pages (from-to)8480-8486
Number of pages7
JournalJournal of the American Chemical Society
Volume134
Issue number20
DOIs
Publication statusPublished - 23 May 2012

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    Milroy, L. G., Rizzo, S., Calderon, A., Ellinger, B., Erdmann, S., Mondry, J., Verveer, P., Bastiaens, P., Waldmann, H., Dehmelt, L., & Arndt, H. D. (2012). Selective chemical imaging of static actin in live cells. Journal of the American Chemical Society, 134(20), 8480-8486. https://doi.org/10.1021/ja211708z