In this study two different types of nano-silica (nS) were applied in self-compacting concrete (SCC), both having similar particle size distributions (PSD), but produced through two different processes: fumed powder silica and precipitated silica in colloidal suspension. The influence of nano-silica on SCC was investigated with respect to the properties of concrete in fresh (workability) and hardened state (mechanical properties and durability). Additionally, the densification of the microstructure of the hardened concrete was verified by SEM and EDS analyses. The obtained results demonstrate that nano-silica efficiently used in SCC can improve its mechanical properties and durability. Considering the reactivity of the two applied nano-silicas, the colloidal type showed a higher reactivity at early age, which influenced the final SCC properties.