Reversibly triggered protein-ligand assemblies in giant vesicles

R.J.R.W. Peters; M. Nijemeisland; J.C.M. van Hest

doi:10.1002/anie.201502920

Reversibly triggered protein-ligand assemblies in giant vesicles

R.J.R.W. Peters
, M. Nijemeisland
, J.C.M. van Hest

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

128 Downloads (Pure)

Abstract

External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.

Original language	English
Pages (from-to)	9614-9617
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	33
DOIs	https://doi.org/10.1002/anie.201502920
Publication status	Published - 1 Aug 2015
Externally published	Yes

Keywords

cell mimic
liposomes
protein-ligand interactions
vesicles

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final_Reversibly triggered protein-ligand assemblies in giant vesicles_AngewChemFinal author version , 337 KB

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KW - cell mimic

KW - liposomes

KW - protein-ligand interactions

KW - vesicles

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Reversibly triggered protein-ligand assemblies in giant vesicles

Abstract

Keywords

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