Repeated fractional intradermal dosing of an inactivated polio vaccine by a single hollow microneedle leads to superior immune responses

P. Schipper, K. van der Maaden, S. Romeijn, C.W.J. Oomens, G. Kersten, W. Jiskoot, J. Bouwstra

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effect of various repeated fractional intradermal dosing schedules of inactivated polio vaccine serotype 1 (IPV1) on IPV1-specific IgG responses in rats. By utilizing an applicator that allowed for precisely controlled intradermal microinjections by using a single hollow microneedle, rats were immunized intradermally with 5 D-antigen units (DU) of IPV1 at 150 μm skin depth. This dose was administered as a bolus, or in a repeated fractional dosing schedule: 4 doses of 1.25 DU (1/4th of total dose) were administered on four consecutive days or every other day; 8 doses of 0.625 DU (1/8th of total dose) were administered on eight consecutive days; or 4 exponentially increasing doses (0.04, 0.16, 0.8 and 4 DU), either with or without an exponentially increasing CpG oligodeoxynucleotide 1826 (CpG) dose, were administered on four consecutive days. All of these fractional dosing schedules resulted in up to ca. 10-fold higher IPV1-specific IgG responses than intradermal and intramuscular bolus dosing. IPV1 combined with adjuvant CpG in exponential dosing did not significantly increase the IPV1-specific IgG responses further, which demonstrated that maximal responses were achieved by fractional dosing. In conclusion, repeated fractional intradermal IPV1 dosing leads to superior IPV1-specific IgG responses without the use of adjuvants. These results indicate that a controlled release delivery system for intradermal IPV1 delivery can potentiate IPV1-specific IgG responses.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalJournal of Controlled Release
Volume242
DOIs
Publication statusPublished - 28 Nov 2016

Keywords

  • Adjuvant
  • Dose-sparing
  • Fractional dose
  • Hollow microneedles
  • Inactivated polio vaccine
  • Intradermal immunization
  • Poliomyelitis

Fingerprint Dive into the research topics of 'Repeated fractional intradermal dosing of an inactivated polio vaccine by a single hollow microneedle leads to superior immune responses'. Together they form a unique fingerprint.

Cite this