Renal epithelial monolayer formation on monomeric and polymeric catechol functionalized supramolecular biomaterials

Ronald C. van Gaal, Michele Fedecostante, Peter Paul K.H. Fransen, Rosalinde Masereeuw, Patricia Y.W. Dankers (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
3 Downloads (Pure)

Abstract

Induction of a functional, tight monolayer of renal epithelial cells on a synthetic membrane to be applied in a bioartificial kidney device requires for bio-activation of the membrane. The current golden standard in bio-activation is the combination of a random polymeric catechol (L-DOPA) coating and collagen type IV (Col IV). Here the possibility of replacing this with defined monomeric catechol functionalization on a biomaterial surface using supramolecular ureido-pyrimidinone (UPy)-moieties is investigated. Monomeric catechols modified with a UPy-unit are successfully incorporated and presented in supramolecular UPy-polymer films and membranes. Unfortunately, these UPy-catechols are unable to improve epithelial cell monolayer formation over time, solely or in combination with Col IV. L-DOPA combined with Col IV is able to induce a tight monolayer capable of transport on electrospun supramolecular UPy-membranes. This study shows that a random polymeric catechol coating cannot be simply mimicked by defined monomeric catechols as supramolecular additives. There is still a long way to go in order to synthetically mimic simple natural structures.

Original languageEnglish
Article number1800300
Number of pages7
JournalMacromolecular Bioscience
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 2019

Keywords

  • bio-artificial kidney
  • catechols
  • renal epithelial cells
  • supramolecular biomaterials
  • ureido-pyrimidinones
  • Tissue Engineering/methods
  • Epithelial Cells/cytology
  • Humans
  • Cells, Cultured
  • Kidneys, Artificial
  • Cell Culture Techniques/instrumentation
  • Biocompatible Materials/pharmacology
  • Cell Adhesion
  • Bioartificial Organs
  • Macromolecular Substances
  • Kidney Tubules, Proximal/cytology
  • Catechols/pharmacology
  • Polymers/pharmacology
  • Tissue Scaffolds/chemistry
  • Pyrimidinones/chemistry
  • Cell Proliferation/drug effects

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