Renal epithelial monolayer formation on monomeric and polymeric catechol functionalized supramolecular biomaterials

Ronald C. van Gaal, Michele Fedecostante, Peter Paul K.H. Fransen, Rosalinde Masereeuw, Patricia Y.W. Dankers (Corresponding author)

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Induction of a functional, tight monolayer of renal epithelial cells on a synthetic membrane to be applied in a bioartificial kidney device requires for bio-activation of the membrane. The current golden standard in bio-activation is the combination of a random polymeric catechol (L-DOPA) coating and collagen type IV (Col IV). Here the possibility of replacing this with defined monomeric catechol functionalization on a biomaterial surface using supramolecular ureido-pyrimidinone (UPy)-moieties is investigated. Monomeric catechols modified with a UPy-unit are successfully incorporated and presented in supramolecular UPy-polymer films and membranes. Unfortunately, these UPy-catechols are unable to improve epithelial cell monolayer formation over time, solely or in combination with Col IV. L-DOPA combined with Col IV is able to induce a tight monolayer capable of transport on electrospun supramolecular UPy-membranes. This study shows that a random polymeric catechol coating cannot be simply mimicked by defined monomeric catechols as supramolecular additives. There is still a long way to go in order to synthetically mimic simple natural structures.

Original languageEnglish
Article number1800300
Number of pages7
JournalMacromolecular Bioscience
Issue number2
Publication statusPublished - Feb 2019


  • bio-artificial kidney
  • catechols
  • renal epithelial cells
  • supramolecular biomaterials
  • ureido-pyrimidinones
  • Tissue Engineering/methods
  • Epithelial Cells/cytology
  • Humans
  • Cells, Cultured
  • Kidneys, Artificial
  • Cell Culture Techniques/instrumentation
  • Biocompatible Materials/pharmacology
  • Cell Adhesion
  • Bioartificial Organs
  • Macromolecular Substances
  • Kidney Tubules, Proximal/cytology
  • Catechols/pharmacology
  • Polymers/pharmacology
  • Tissue Scaffolds/chemistry
  • Pyrimidinones/chemistry
  • Cell Proliferation/drug effects


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