Refining the design of diblock elastin-like polypeptides for self-assembly into nanoparticles

Michèle Dai; Evangelos Georgilis; Guillaume Goudounet; Bertrand Garbay; Jan Pille; Jan C.M. van Hest; Xavier Schultze; Elisabeth Garanger; Sébastien Lecommandoux

doi:10.3390/polym13091470

Refining the design of diblock elastin-like polypeptides for self-assembly into nanoparticles

Michèle Dai, Evangelos Georgilis, Guillaume Goudounet, Bertrand Garbay, Jan Pille, Jan C.M. van Hest, Xavier Schultze, Elisabeth Garanger (Corresponding author), Sébastien Lecommandoux (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

20 Citations (Scopus)

Abstract

Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M₁V₃-i]-[I-j]. First, ELP-[M₁V₃-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M₁V₃-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M₁V₃-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.

Original language	English
Article number	1470
Number of pages	17
Journal	Polymers
Volume	13
Issue number	9
DOIs	https://doi.org/10.3390/polym13091470
Publication status	Published - 1 May 2021

Funding

Funding: This work was supported by a CIFRE grant to M.D. from ANRT and by the European Union’s Horizon 2020 research and innovation program Marie Skłodowska-Curie Innovative Training Networks (ITN) under grant No. 676137 (NANOMED project).

Funders	Funder number
Marie Skłodowska‐Curie
European Union's Horizon 2020 - Research and Innovation Framework Programme	676137

Keywords

Elastin-like polypeptides
Methionine oxidation
Nanoparticles
Self-assembly
Thermoresponsiveness

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10.3390/polym13091470

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abstract = "Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M1V3-i]-[I-j]. First, ELP-[M1V3-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M1V3-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M1V3-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.",

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AU - Georgilis, Evangelos

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AU - Pille, Jan

AU - van Hest, Jan C.M.

AU - Schultze, Xavier

AU - Garanger, Elisabeth

AU - Lecommandoux, Sébastien

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N2 - Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M1V3-i]-[I-j]. First, ELP-[M1V3-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M1V3-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M1V3-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.

AB - Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M1V3-i]-[I-j]. First, ELP-[M1V3-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M1V3-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M1V3-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.

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KW - Thermoresponsiveness

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Refining the design of diblock elastin-like polypeptides for self-assembly into nanoparticles

Abstract

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Keywords

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