Abstract
Industrial food processes are monitored to ensure that food is being produced with good quality, yield, and productivity. For developing innovative real-time monitoring and control strategies, real-time sensors are needed that can continuously report chemical and biochemical data of the manufacturing process. Here, we describe a generalizable methodology to develop affinity-based biosensors for the continuous monitoring of small molecules in industrial food processes. Phage-display antibody fragments were developed for the measurement of small molecules, as exemplified with the measurement of glycoalkaloids (GAs) in potato fruit juice. The recombinant antibodies were selected for use in a competition-based biosensor with single-molecule resolution, called biosensing by particle motion, using assay architectures with free particles as well as tethered particles. The resulting sensor measures GAs in the micromolar range, is reversible, has a measurement response time below 5 min, and enables continuous monitoring of GAs in protein-rich solutions for more than 20 h with concentration measurement errors below 15%. The demonstrated biosensor gives the perspective to enable a variety of monitoring and control strategies based on continuous measurement of small molecules in industrial food processes.
| Original language | English |
|---|---|
| Pages (from-to) | 7950-7959 |
| Number of pages | 10 |
| Journal | Analytical Chemistry |
| Volume | 95 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 23 May 2023 |
Funding
We thank Avebe for kindly providing the PFJ samples and René Hoet (Maastricht University, Department of Pathology) for critically reading the manuscript. This work was partly funded by The Netherlands Topsectors Agri&Food, HTSM, and Chemistry under contract number LWV20.117.