Rapid inorganic ion analysis using quantitative microchip capillary electrophoresis

E.X. Vrouwe, R. Luttge, W. Olthuis, A. van den Berg

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Rapid quantitative microchip capillary electrophoresis (CE) for online monitoring of drinking water enabling inorganic ion separation in less than 15 s is presented. Comparing cationic and anionic standards at different concentrations the analysis of cationic species resulted in non-linear calibration curves. We interpret this effect as a variation in the volume of the injected sample plug caused by changes of the electroosmotic flow (EOF) due to the strong interaction of bivalent cations with the glass surface. This explanation is supported by the observation of severe peak tailing. Conducting microchip CE analysis in a glass microchannel, optimized conditions are received for the cationic species K+, Na+, Ca2+, Mg2+ using a background electrolyte consisting of 30 mmol/L histidine and 2-(N-morpholino)ethanesulfonic acid, containing 0.5 mmol/L potassium chloride to reduce surface interaction and 4 mmol/L tartaric acid as a complexing agent resulting in a pH-value of 5.8. Applying reversed EOF co-migration for the anionic species Cl-, SO42- and HCO3- optimized separation occurs in a background electrolyte consisting of 10 mmol/L 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) and 10 mmol/L HEPES sodium salt, containing 0.05 mmol/L CTAB (cetyltrimethylammonium bromide) resulting in a pH-value of 7.5. The detection limits are 20 µmol/L for the monovalent cationic and anionic species and 10 µmol/L for the divalent species. These values make the method very suitable for many applications including the analysis of abundant ions in tap water as demonstrated in this paper. © 2006 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalJournal of Chromatography, A
Volume1102
Issue number1-2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Microchip Electrophoresis
Electroosmosis
Capillary electrophoresis
Capillary Electrophoresis
Electrolytes
Glass
Ions
HEPES
Potassium Chloride
Tailings
Microchannels
Chemical analysis
Histidine
Drinking Water
Calibration
Limit of Detection
Cations
Salts
Sodium
Observation

Cite this

Vrouwe, E.X. ; Luttge, R. ; Olthuis, W. ; van den Berg, A. / Rapid inorganic ion analysis using quantitative microchip capillary electrophoresis. In: Journal of Chromatography, A. 2006 ; Vol. 1102, No. 1-2. pp. 287-293.
@article{e6e91715b72f4f48b70c943889ee8301,
title = "Rapid inorganic ion analysis using quantitative microchip capillary electrophoresis",
abstract = "Rapid quantitative microchip capillary electrophoresis (CE) for online monitoring of drinking water enabling inorganic ion separation in less than 15 s is presented. Comparing cationic and anionic standards at different concentrations the analysis of cationic species resulted in non-linear calibration curves. We interpret this effect as a variation in the volume of the injected sample plug caused by changes of the electroosmotic flow (EOF) due to the strong interaction of bivalent cations with the glass surface. This explanation is supported by the observation of severe peak tailing. Conducting microchip CE analysis in a glass microchannel, optimized conditions are received for the cationic species K+, Na+, Ca2+, Mg2+ using a background electrolyte consisting of 30 mmol/L histidine and 2-(N-morpholino)ethanesulfonic acid, containing 0.5 mmol/L potassium chloride to reduce surface interaction and 4 mmol/L tartaric acid as a complexing agent resulting in a pH-value of 5.8. Applying reversed EOF co-migration for the anionic species Cl-, SO42- and HCO3- optimized separation occurs in a background electrolyte consisting of 10 mmol/L 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) and 10 mmol/L HEPES sodium salt, containing 0.05 mmol/L CTAB (cetyltrimethylammonium bromide) resulting in a pH-value of 7.5. The detection limits are 20 µmol/L for the monovalent cationic and anionic species and 10 µmol/L for the divalent species. These values make the method very suitable for many applications including the analysis of abundant ions in tap water as demonstrated in this paper. {\circledC} 2006 Elsevier B.V. All rights reserved.",
author = "E.X. Vrouwe and R. Luttge and W. Olthuis and {van den Berg}, A.",
year = "2006",
doi = "10.1016/j.chroma.2005.10.064",
language = "English",
volume = "1102",
pages = "287--293",
journal = "Journal of Chromatography, A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

Rapid inorganic ion analysis using quantitative microchip capillary electrophoresis. / Vrouwe, E.X.; Luttge, R.; Olthuis, W.; van den Berg, A.

In: Journal of Chromatography, A, Vol. 1102, No. 1-2, 2006, p. 287-293.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Rapid inorganic ion analysis using quantitative microchip capillary electrophoresis

AU - Vrouwe, E.X.

AU - Luttge, R.

AU - Olthuis, W.

AU - van den Berg, A.

PY - 2006

Y1 - 2006

N2 - Rapid quantitative microchip capillary electrophoresis (CE) for online monitoring of drinking water enabling inorganic ion separation in less than 15 s is presented. Comparing cationic and anionic standards at different concentrations the analysis of cationic species resulted in non-linear calibration curves. We interpret this effect as a variation in the volume of the injected sample plug caused by changes of the electroosmotic flow (EOF) due to the strong interaction of bivalent cations with the glass surface. This explanation is supported by the observation of severe peak tailing. Conducting microchip CE analysis in a glass microchannel, optimized conditions are received for the cationic species K+, Na+, Ca2+, Mg2+ using a background electrolyte consisting of 30 mmol/L histidine and 2-(N-morpholino)ethanesulfonic acid, containing 0.5 mmol/L potassium chloride to reduce surface interaction and 4 mmol/L tartaric acid as a complexing agent resulting in a pH-value of 5.8. Applying reversed EOF co-migration for the anionic species Cl-, SO42- and HCO3- optimized separation occurs in a background electrolyte consisting of 10 mmol/L 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) and 10 mmol/L HEPES sodium salt, containing 0.05 mmol/L CTAB (cetyltrimethylammonium bromide) resulting in a pH-value of 7.5. The detection limits are 20 µmol/L for the monovalent cationic and anionic species and 10 µmol/L for the divalent species. These values make the method very suitable for many applications including the analysis of abundant ions in tap water as demonstrated in this paper. © 2006 Elsevier B.V. All rights reserved.

AB - Rapid quantitative microchip capillary electrophoresis (CE) for online monitoring of drinking water enabling inorganic ion separation in less than 15 s is presented. Comparing cationic and anionic standards at different concentrations the analysis of cationic species resulted in non-linear calibration curves. We interpret this effect as a variation in the volume of the injected sample plug caused by changes of the electroosmotic flow (EOF) due to the strong interaction of bivalent cations with the glass surface. This explanation is supported by the observation of severe peak tailing. Conducting microchip CE analysis in a glass microchannel, optimized conditions are received for the cationic species K+, Na+, Ca2+, Mg2+ using a background electrolyte consisting of 30 mmol/L histidine and 2-(N-morpholino)ethanesulfonic acid, containing 0.5 mmol/L potassium chloride to reduce surface interaction and 4 mmol/L tartaric acid as a complexing agent resulting in a pH-value of 5.8. Applying reversed EOF co-migration for the anionic species Cl-, SO42- and HCO3- optimized separation occurs in a background electrolyte consisting of 10 mmol/L 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) and 10 mmol/L HEPES sodium salt, containing 0.05 mmol/L CTAB (cetyltrimethylammonium bromide) resulting in a pH-value of 7.5. The detection limits are 20 µmol/L for the monovalent cationic and anionic species and 10 µmol/L for the divalent species. These values make the method very suitable for many applications including the analysis of abundant ions in tap water as demonstrated in this paper. © 2006 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.chroma.2005.10.064

DO - 10.1016/j.chroma.2005.10.064

M3 - Article

C2 - 16310794

VL - 1102

SP - 287

EP - 293

JO - Journal of Chromatography, A

JF - Journal of Chromatography, A

SN - 0021-9673

IS - 1-2

ER -