Raman microscopy of cells: Chemical imaging of apoptosis

Natallia Uzunbajakava, Jan Greve, Gees Otto

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)

Abstract

Singular value decomposition was applied to the set of the non-resonant Raman spectra, recorded during Raman imaging of the single apoptotic cell. The basis vectors and the corresponding singular values were assessed in terms of their statistical significance. The noise-containing basis vectors were rejected while keeping the meaningful ones. In this way the Raman images of the apoptotic cell were successfully reconstructed from the spectrally-filtered data matrix. The results are demonstrated on the spatial distribution of the DNA, protein and phospholipids, present in the fragments of the apoptotic cell.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4963
DOIs
Publication statusPublished - 2003
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States
Duration: 26 Jan 200328 Jan 2003

Keywords

  • Apoptosis
  • DNA
  • Phospholipids
  • Protein
  • Raman imaging

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