RAFT Emulsion Polymerization as a Platform to Generate Well-Defined Biocompatible Latex Nanoparticles

Pratik Gurnani; Carlos Sanchez-Cano; Kristin Abraham; Helena Xandri-Monje; Alexander B. Cook; Matthias Hartlieb; Francis Lévi; Robert Dallmann; Sébastien Perrier

doi:10.1002/mabi.201800213

RAFT Emulsion Polymerization as a Platform to Generate Well-Defined Biocompatible Latex Nanoparticles

Pratik Gurnani, Carlos Sanchez-Cano, Kristin Abraham, Helena Xandri-Monje, Alexander B. Cook, Matthias Hartlieb, Francis Lévi, Robert Dallmann, Sébastien Perrier

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circulatory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface composition and were further characterized with light scattering, electron microscopy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any purification after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.

Original language	English
Article number	1800213
Journal	Macromolecular Bioscience
Volume	18
Issue number	10
DOIs	https://doi.org/10.1002/mabi.201800213
Publication status	Published - Oct 2018
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank CRUK/EPSRC (C53561/A19933; P.G., C.S.-C., K.A., H.X.-M., F.L., S.P.), the German Research Foundation (DFG, GZ; HA 7725/1-1; M.H.), the Warwick Innovation Fund (R.D.), and the Royal Society Wolfson Merit Award (WM130055; S.P.) for financial support. The authors are grateful to LICOR Inc. for access to a Pearl Trilogy imaging system for in vivo experiments. The staff of the BSU at Warwick are thanked for animal husbandry.

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

biocompatible nanoparticles
core–shell
reversible addition fragmentation chain transfer emulsion polymerization
tunable nanoparticles
Caco-2 Cells
Emulsions
Humans
Polymerization
Male
Materials Testing
Latex/chemistry
Animals
Nanoparticles/chemistry
Mice

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10.1002/mabi.201800213

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title = "RAFT Emulsion Polymerization as a Platform to Generate Well-Defined Biocompatible Latex Nanoparticles",

abstract = "Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circulatory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface composition and were further characterized with light scattering, electron microscopy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any purification after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.",

keywords = "biocompatible nanoparticles, core–shell, reversible addition fragmentation chain transfer emulsion polymerization, tunable nanoparticles, Caco-2 Cells, Emulsions, Humans, Polymerization, Male, Materials Testing, Latex/chemistry, Animals, Nanoparticles/chemistry, Mice",

author = "Pratik Gurnani and Carlos Sanchez-Cano and Kristin Abraham and Helena Xandri-Monje and Cook, {Alexander B.} and Matthias Hartlieb and Francis L{\'e}vi and Robert Dallmann and S{\'e}bastien Perrier",

note = "Funding Information: The authors thank CRUK/EPSRC (C53561/A19933; P.G., C.S.-C., K.A., H.X.-M., F.L., S.P.), the German Research Foundation (DFG, GZ; HA 7725/1-1; M.H.), the Warwick Innovation Fund (R.D.), and the Royal Society Wolfson Merit Award (WM130055; S.P.) for financial support. The authors are grateful to LICOR Inc. for access to a Pearl Trilogy imaging system for in vivo experiments. The staff of the BSU at Warwick are thanked for animal husbandry. Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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doi = "10.1002/mabi.201800213",

language = "English",

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AU - Gurnani, Pratik

AU - Sanchez-Cano, Carlos

AU - Abraham, Kristin

AU - Xandri-Monje, Helena

AU - Cook, Alexander B.

AU - Hartlieb, Matthias

AU - Lévi, Francis

AU - Dallmann, Robert

AU - Perrier, Sébastien

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N2 - Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circulatory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface composition and were further characterized with light scattering, electron microscopy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any purification after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.

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RAFT Emulsion Polymerization as a Platform to Generate Well-Defined Biocompatible Latex Nanoparticles

Abstract

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Keywords

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