Quantitative assessment of birefringent skin structures in scattered light confocal imaging using radially polarized light

Babu Varghese, Rieko Verhagen, Altaf Hussain, Clemence Boudot, Qiangqiang Tai, Siqi Ding, Jasmin A. Holz, Natallia E. Uzunbajakava

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

The polarization characteristics of birefringent tissues could be only partially obtained using linearly polarized light in polarization sensitive optical imaging. Here we analyze the change in polarization of backscattered light from birefringent structures versus the orientations of the incident polarizations using linearly, circularly and radially polarized light in a cross-polarized confocal microscope. A spatially variable retardation plate composed of eight sectors of λ/2 wave plates was used to transform linearly polarized light into a radially polarized light. Based on the experimental data obtained from ex-vivo measurements on human scalp hairs and in-vivo measurements on hair and skin, we exemplify that the underestimation of the birefringence content resulting from the orientation related effects associated with the use of linearly polarized light for imaging tissues containing wavy birefringent structures could be minimized by using radially polarized light.

Original languageEnglish
Pages (from-to)12527-12535
Number of pages9
JournalSensors (Switzerland)
Volume13
Issue number9
DOIs
Publication statusPublished - 17 Sep 2013
Externally publishedYes

Keywords

  • Birefringence
  • Confocal microscopy
  • Polarization dependent contrast
  • Radial polarization

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