Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution

A. Van Reenen; A.M. De Jong; M. W J Prins

Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution

A. Van Reenen, A.M. De Jong, M. W J Prins

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The kinetics of affinity capturing of fluorescent nanoparticles by magnetic microparticles is investigated for biosensing purposes. The capturing of nanoparticles is recorded using fluorescence microscopy with single target resolution. Comparing the binding kinetics by diffusion versus fluid vortex mixing, a six times enhancement of the reaction rate constant is observed, which implies that transport plays a significant role in the capturing process. The results represent a benchmark for further investigations on controlled mixing and target catching using actuated magnetic particles for biosensing.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	1627-1629
Number of pages	3
Volume	3
Publication status	Published - 1 Dec 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011 - Washington State Convention Center, Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 http://microtasconferences.org/microtas2011/

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011
Abbreviated title	MicroTAS 2011
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	http://microtasconferences.org/microtas2011/

Bibliographical note

Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), 2-6 October 2011, Seattle, Washington

Keywords

Biosensing
Mixing
Particles
Reaction kinetics

Cite this

@inproceedings{caa8ae5f85914ad9b02c7ffe56c918fd,

title = "Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution",

abstract = "The kinetics of affinity capturing of fluorescent nanoparticles by magnetic microparticles is investigated for biosensing purposes. The capturing of nanoparticles is recorded using fluorescence microscopy with single target resolution. Comparing the binding kinetics by diffusion versus fluid vortex mixing, a six times enhancement of the reaction rate constant is observed, which implies that transport plays a significant role in the capturing process. The results represent a benchmark for further investigations on controlled mixing and target catching using actuated magnetic particles for biosensing.",

keywords = "Biosensing, Mixing, Particles, Reaction kinetics",

author = "{Van Reenen}, A. and {De Jong}, A.M. and Prins, {M. W J}",

note = "Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), 2-6 October 2011, Seattle, Washington; 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

year = "2011",

month = dec,

day = "1",

language = "English",

isbn = "9781618395955",

volume = "3",

pages = "1627--1629",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

url = "http://microtasconferences.org/microtas2011/",

}

Van Reenen, A, De Jong, AM & Prins, MWJ 2011, Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1627-1629, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011, Seattle, Washington, United States, 2/10/11.

Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution. / Van Reenen, A.; De Jong, A.M.; Prins, M. W J.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1627-1629.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution

AU - Van Reenen, A.

AU - De Jong, A.M.

AU - Prins, M. W J

N1 - Conference code: 15

PY - 2011/12/1

Y1 - 2011/12/1

N2 - The kinetics of affinity capturing of fluorescent nanoparticles by magnetic microparticles is investigated for biosensing purposes. The capturing of nanoparticles is recorded using fluorescence microscopy with single target resolution. Comparing the binding kinetics by diffusion versus fluid vortex mixing, a six times enhancement of the reaction rate constant is observed, which implies that transport plays a significant role in the capturing process. The results represent a benchmark for further investigations on controlled mixing and target catching using actuated magnetic particles for biosensing.

AB - The kinetics of affinity capturing of fluorescent nanoparticles by magnetic microparticles is investigated for biosensing purposes. The capturing of nanoparticles is recorded using fluorescence microscopy with single target resolution. Comparing the binding kinetics by diffusion versus fluid vortex mixing, a six times enhancement of the reaction rate constant is observed, which implies that transport plays a significant role in the capturing process. The results represent a benchmark for further investigations on controlled mixing and target catching using actuated magnetic particles for biosensing.

KW - Biosensing

KW - Mixing

KW - Particles

KW - Reaction kinetics

UR - http://www.scopus.com/inward/record.url?scp=84883768334&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781618395955

VL - 3

SP - 1627

EP - 1629

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution

Abstract

Conference

Bibliographical note

Keywords

Other files and links

Fingerprint

Cite this