Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution

A. Van Reenen, A.M. De Jong, M. W J Prins

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)

Abstract

The kinetics of affinity capturing of fluorescent nanoparticles by magnetic microparticles is investigated for biosensing purposes. The capturing of nanoparticles is recorded using fluorescence microscopy with single target resolution. Comparing the binding kinetics by diffusion versus fluid vortex mixing, a six times enhancement of the reaction rate constant is observed, which implies that transport plays a significant role in the capturing process. The results represent a benchmark for further investigations on controlled mixing and target catching using actuated magnetic particles for biosensing.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1627-1629
Number of pages3
Volume3
Publication statusPublished - 1 Dec 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAs 2011) - Washington State Convention Center, Seattle, United States
Duration: 2 Oct 20116 Oct 2011
Conference number: 15
http://microtasconferences.org/microtas2011/

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAs 2011)
Abbreviated titleMicroTAs 2011
CountryUnited States
CitySeattle
Period2/10/116/10/11
Internet address

Bibliographical note

Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), 2-6 October 2011, Seattle, Washington

Keywords

  • Biosensing
  • Mixing
  • Particles
  • Reaction kinetics

Fingerprint Dive into the research topics of 'Quantification of the mix and catch efficiency by microparticles for biosensing with single-molecule resolution'. Together they form a unique fingerprint.

Cite this