Quantification of skeletal muscle mitochondrial function by 31P magnetic resonance spectroscopy techniques: A quantitative review

G.J. Kemp, R.E. Ahmad, K. Nicolay, J.J. Prompers

    Research output: Contribution to journalArticleAcademicpeer-review

    91 Citations (Scopus)
    2 Downloads (Pure)

    Abstract

    Magnetic resonance spectroscopy (MRS) can give information about cellular metabolism in vivo which is difficult to obtain in other ways. In skeletal muscle, non-invasive 31P MRS measurements of the post-exercise recovery kinetics of pH, [PCr], [Pi] and [ADP] contain valuable information about muscle mitochondrial function and cellular pH homeostasis in vivo, but quantitative interpretation depends on understanding the underlying physiology. Here, by giving examples of the analysis of 31P MRS recovery data, by some simple computational simulation, and by extensively comparing data from published studies using both 31P MRS and invasive direct measurements of muscle O2 consumption in a common analytical framework, we consider what can be learnt quantitatively about mitochondrial metabolism in skeletal muscle using MRS-based methodology. We explore some technical and conceptual limitations of current methods, and point out some aspects of the physiology which are still incompletely understood.

    Original languageEnglish
    Pages (from-to)107-144
    Number of pages38
    JournalActa Physiologica
    Volume213
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2015

    Keywords

    • P magnetic resonance spectroscopy
    • Metabolism
    • Mitochondria
    • Non-invasive methods
    • Oxygen consumption
    • Skeletal muscle

    Fingerprint

    Dive into the research topics of 'Quantification of skeletal muscle mitochondrial function by 31P magnetic resonance spectroscopy techniques: A quantitative review'. Together they form a unique fingerprint.

    Cite this