Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633

H.F.M. Hermes; T. Sonke; P.J.H. Peters; J.A.M. Balken, van; J. Kamphuis; L. Dijkhuizen; E.M. Meijer

Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633

H.F.M. Hermes, T. Sonke, P.J.H. Peters, J.A.M. Balken, van, J. Kamphuis, L. Dijkhuizen, E.M. Meijer

Macromolecular and Organic Chemistry

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Abstract

An L-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of D,L-amino acid amide racemates, was purified to homogeneity. The highly L-enantioselective enzyme resembled thiol reagent-sensitive alk. serine proteinases was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and a-H amino acid amides, e.g., L-phenylglycine amide. [on SciFinder (R)]

Original language	English
Pages (from-to)	4330-4334
Journal	Applied and Environmental Microbiology
Volume	59
Issue number	12
Publication status	Published - 1993

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title = "Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633",

abstract = "An L-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of D,L-amino acid amide racemates, was purified to homogeneity. The highly L-enantioselective enzyme resembled thiol reagent-sensitive alk. serine proteinases was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and a-H amino acid amides, e.g., L-phenylglycine amide. [on SciFinder (R)]",

author = "H.F.M. Hermes and T. Sonke and P.J.H. Peters and {Balken, van}, J.A.M. and J. Kamphuis and L. Dijkhuizen and E.M. Meijer",

year = "1993",

language = "English",

volume = "59",

pages = "4330--4334",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "12",

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TY - JOUR

T1 - Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633

AU - Hermes, H.F.M.

AU - Sonke, T.

AU - Peters, P.J.H.

AU - Balken, van, J.A.M.

AU - Kamphuis, J.

AU - Dijkhuizen, L.

AU - Meijer, E.M.

PY - 1993

Y1 - 1993

N2 - An L-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of D,L-amino acid amide racemates, was purified to homogeneity. The highly L-enantioselective enzyme resembled thiol reagent-sensitive alk. serine proteinases was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and a-H amino acid amides, e.g., L-phenylglycine amide. [on SciFinder (R)]

AB - An L-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of D,L-amino acid amide racemates, was purified to homogeneity. The highly L-enantioselective enzyme resembled thiol reagent-sensitive alk. serine proteinases was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and a-H amino acid amides, e.g., L-phenylglycine amide. [on SciFinder (R)]

M3 - Article

SN - 0099-2240

VL - 59

SP - 4330

EP - 4334

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 12

ER -

Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633

Abstract

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