Protein modification by strain-promoted alkyne-azide cycloaddition

J.C.M. Hest, van, F.L. van Delft

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)


Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.

Original languageEnglish
Pages (from-to)1309-1312
Number of pages4
Issue number9
Publication statusPublished - 14 Jun 2011
Externally publishedYes


  • Azides
  • Bioorthogonal chemistry
  • Cycloadditions
  • Cyclooctynes
  • Protein modification

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