Protein modification by strain-promoted alkyne-azide cycloaddition

J.C.M. Hest, van, F.L. van Delft

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.

Original languageEnglish
Pages (from-to)1309-1312
Number of pages4
JournalChemBioChem
Volume12
Issue number9
DOIs
Publication statusPublished - 14 Jun 2011
Externally publishedYes

Fingerprint

Alkynes
Azides
Cycloaddition
Cycloaddition Reaction
Proteins
Genetic Techniques
Ecosystem

Keywords

  • Azides
  • Bioorthogonal chemistry
  • Cycloadditions
  • Cyclooctynes
  • Protein modification

Cite this

Hest, van, J.C.M. ; van Delft, F.L. / Protein modification by strain-promoted alkyne-azide cycloaddition. In: ChemBioChem. 2011 ; Vol. 12, No. 9. pp. 1309-1312.
@article{0630dc4a51a6407fb1b2e1cc611b14e7,
title = "Protein modification by strain-promoted alkyne-azide cycloaddition",
abstract = "Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.",
keywords = "Azides, Bioorthogonal chemistry, Cycloadditions, Cyclooctynes, Protein modification",
author = "{Hest, van}, J.C.M. and {van Delft}, F.L.",
year = "2011",
month = "6",
day = "14",
doi = "10.1002/cbic.201100206",
language = "English",
volume = "12",
pages = "1309--1312",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "9",

}

Protein modification by strain-promoted alkyne-azide cycloaddition. / Hest, van, J.C.M.; van Delft, F.L.

In: ChemBioChem, Vol. 12, No. 9, 14.06.2011, p. 1309-1312.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Protein modification by strain-promoted alkyne-azide cycloaddition

AU - Hest, van, J.C.M.

AU - van Delft, F.L.

PY - 2011/6/14

Y1 - 2011/6/14

N2 - Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.

AB - Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.

KW - Azides

KW - Bioorthogonal chemistry

KW - Cycloadditions

KW - Cyclooctynes

KW - Protein modification

UR - http://www.scopus.com/inward/record.url?scp=79958763426&partnerID=8YFLogxK

U2 - 10.1002/cbic.201100206

DO - 10.1002/cbic.201100206

M3 - Article

C2 - 21557431

AN - SCOPUS:79958763426

VL - 12

SP - 1309

EP - 1312

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 9

ER -