Properties of cyclodextrins. IV. Features and use of insoluble cyclodextrin-epichlorohydrin resins

Cyclodextrin-epichlorohydrin resins, prepd. by crosslinking a-cyclodextrin or b-cyclodextrin (I) with 10-25% epichlorohydrin (II), gave results comparable with those obtained with Sephadex G-25 coarse when both resins were used for gel chromatog., i.e., designated as gel inclusion chromatog. because cyclodextrin voids form inclusion compds. of different stabilities with the substances to be sepd. A 17:3 I-II resin had slightly greater thermal stability than the Sephadex G-25. The elution vols. on a column of 3:1 I-II resin eluted with 20 vol. % aq. EtOH were as follows (compd., elution vol. in ml. for the I-II resin and Sephadex G-25, resp., given): glyceryl L-tyrosine, 142, 120; DL-benzoylalanine, 47, 60; salicine, 104, 110; L-tyrosine HCl 105, 135; DL-tryptophan HCl, 207, 208; aniline HCl, 511, 150; BzOH, 400, 100; phenol, does not leave the column, 133; o-chlorobenzoic acid, 98, 83; m-chlorobenzoic acid, 400, 83; dextran blue, 40, 55. The behavior of resins contg. I differs from that of resins contg. a-cyclodextrin because the void sizes are different. [on SciFinder (R)]