Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase

J.A.M. Balken, van, T.J.G.M. Dooren, van, W.J.J. Twell, van den, J. Kamphuis, E.M. Meijer

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)
169 Downloads (Pure)

Abstract

Favourable reaction conditions for the enzymatic production of 1-kestose by sucrose-1F-fructosyltransferase, SFT (EC 2.4.1.99) from Aspergillus phoenicis CBS 294.80 mycelium were established. The intracellular enzyme SFT works best at 60°C, exhibits a relatively high thermostability and possesses an alkaline pH optimum. An invertase also present in the mycelium of A. phoenicis possesses an acidic pH optimum. Consequently, around pH 8.0 sucrose is converted mainly to 1-kestose and nystose while fructose is only formed in relatively small amounts. Under optimal conditions (55° C, pH 8.0 and an initial sucrose concentration of 750 g 1-1) a yield of about 300 g 1-kestose per 1.01 reaction mixture could be achieved after 8 h.
Original languageEnglish
Pages (from-to)216-221
JournalApplied Microbiology and Biotechnology
Volume35
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Dive into the research topics of 'Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase'. Together they form a unique fingerprint.

Cite this