Product inhibition of papain action

L.A.A.E. Sluyterman

doi:10.1016/0926-6569(64)90252-4

Product inhibition of papain action

L.A.A.E. Sluyterman

Chemical Engineering and Chemistry

Research output: Contribution to journal › Article › Academic

9 Citations (Scopus)

1 Downloads (Pure)

Abstract

Benzoylarginine inhibited papain (EC 3.4.4.10) action competitively in a 1:1 combination of enzyme and inhibitor. The inhibition was small in the range pH 5–8, considerable around pH 4. This pH effect can be explained in terms of the ionization of the carboxyl group of benzoylarginine. Benzoylarginine too is a competitive inhibitor. It inhibited to a lesser extent than benzoylarginine. Several points of similarity are pointed out between the interactions of papain and inhibitor and of papain and substrate.

Original language	English
Pages (from-to)	316-321
Number of pages	6
Journal	Biochimica et Biophysica Acta, Specialized Section on Enzymological Subjects
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/0926-6569(64)90252-4
Publication status	Published - 1964

Access to Document

10.1016/0926-6569(64)90252-4

Cite this

@article{6cb2c25adc1c4422af36c6cebcbea7ae,

title = "Product inhibition of papain action",

abstract = "Benzoylarginine inhibited papain (EC 3.4.4.10) action competitively in a 1:1 combination of enzyme and inhibitor. The inhibition was small in the range pH 5–8, considerable around pH 4. This pH effect can be explained in terms of the ionization of the carboxyl group of benzoylarginine. Benzoylarginine too is a competitive inhibitor. It inhibited to a lesser extent than benzoylarginine. Several points of similarity are pointed out between the interactions of papain and inhibitor and of papain and substrate.",

author = "L.A.A.E. Sluyterman",

year = "1964",

doi = "10.1016/0926-6569(64)90252-4",

language = "English",

volume = "85",

pages = "316--321",

journal = "Biochimica et Biophysica Acta, Specialized Section on Enzymological Subjects",

issn = "0926-6569",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Product inhibition of papain action

AU - Sluyterman, L.A.A.E.

PY - 1964

Y1 - 1964

N2 - Benzoylarginine inhibited papain (EC 3.4.4.10) action competitively in a 1:1 combination of enzyme and inhibitor. The inhibition was small in the range pH 5–8, considerable around pH 4. This pH effect can be explained in terms of the ionization of the carboxyl group of benzoylarginine. Benzoylarginine too is a competitive inhibitor. It inhibited to a lesser extent than benzoylarginine. Several points of similarity are pointed out between the interactions of papain and inhibitor and of papain and substrate.

AB - Benzoylarginine inhibited papain (EC 3.4.4.10) action competitively in a 1:1 combination of enzyme and inhibitor. The inhibition was small in the range pH 5–8, considerable around pH 4. This pH effect can be explained in terms of the ionization of the carboxyl group of benzoylarginine. Benzoylarginine too is a competitive inhibitor. It inhibited to a lesser extent than benzoylarginine. Several points of similarity are pointed out between the interactions of papain and inhibitor and of papain and substrate.

U2 - 10.1016/0926-6569(64)90252-4

DO - 10.1016/0926-6569(64)90252-4

M3 - Article

SN - 0926-6569

VL - 85

SP - 316

EP - 321

JO - Biochimica et Biophysica Acta, Specialized Section on Enzymological Subjects

JF - Biochimica et Biophysica Acta, Specialized Section on Enzymological Subjects

IS - 2

ER -

Product inhibition of papain action

Abstract

Access to Document

Fingerprint

Cite this