Abstract
A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 μm I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column properties, such as morphology, porosity, stability and reproducibility, were extensively tested.
| Original language | English |
|---|---|
| Pages (from-to) | 215-227 |
| Number of pages | 13 |
| Journal | Journal of Chromatography, A |
| Volume | 923 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 20 Jul 2001 |
Keywords
- Electrochromatography
- Micropore
- Monolithic polymer column
- Porosity
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