Preparation and characterization of monolithic polymer columns for capillary electrochromatography

T. Jiang, J. Jiskra, H.A. Claessens, C.A. Cramers

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103 Citations (Scopus)


A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 μm I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column properties, such as morphology, porosity, stability and reproducibility, were extensively tested.

Original languageEnglish
Pages (from-to)215-227
Number of pages13
JournalJournal of Chromatography, A
Issue number1-2
Publication statusPublished - 20 Jul 2001


  • Electrochromatography
  • Micropore
  • Monolithic polymer column
  • Porosity


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