TY - JOUR
T1 - Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes
AU - Fleuren, W.W.M.
AU - Linssen, M.M.L.
AU - Toonen, E.J.M.
AU - Zon, van der, G.C.M.
AU - Guigas, B.
AU - Vlieg, de, J.
AU - Dokter, W.H.A.
AU - Ouwens, D.M.
AU - Alkema, W.
PY - 2013
Y1 - 2013
N2 - Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this are not completely understood. We used a combination of transcriptomics, antibody arrays and bioinformatics approaches to characterize prednisolone-induced alterations in gene expression and adipokine secretion, which could underlie metabolic dysfunction in 3T3-L1 adipocytes. Several pathways, including cytokine signalling, Akt signalling, and Wnt signalling were found to be regulated at multiple levels, showing that these processes are targeted by prednisolone. These results suggest that mechanisms by which prednisolone induce insulin resistance include dysregulation of wnt signalling and immune response processes. These pathways may provide interesting targets for the development of improved glucocorticoids.
Keywords: Gene profiling, glucocorticoids, metabolic dysfunction
AB - Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this are not completely understood. We used a combination of transcriptomics, antibody arrays and bioinformatics approaches to characterize prednisolone-induced alterations in gene expression and adipokine secretion, which could underlie metabolic dysfunction in 3T3-L1 adipocytes. Several pathways, including cytokine signalling, Akt signalling, and Wnt signalling were found to be regulated at multiple levels, showing that these processes are targeted by prednisolone. These results suggest that mechanisms by which prednisolone induce insulin resistance include dysregulation of wnt signalling and immune response processes. These pathways may provide interesting targets for the development of improved glucocorticoids.
Keywords: Gene profiling, glucocorticoids, metabolic dysfunction
U2 - 10.3109/13813455.2013.774022
DO - 10.3109/13813455.2013.774022
M3 - Article
C2 - 23506355
SN - 1381-3455
VL - 119
SP - 52
EP - 64
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 2
ER -
