From kinetic gas theory, it is known that the Prandtl number for hard-sphere monatomic gases is 2/3. Lower values can e realized using gas mixtures of heavy and light monatomic gases. Prandtl numbers varying between 0.2 and 0.67 are obtained by using gas mixtures of helium-argon, helium-krypton, and helium-xenon, This paper presents the results of an experimental investigation into the effect of Prandtl number on the performance of a thermoacoustic refrigerator using gas mixtures. The measurements show that the performance of the refrigerator improves as the Prandtl number decreases. The lowest Prandtl number of 0.2, obtained with a mixture containing 30% xenon. leads to a coefficient of performance relative to Carnot which is 70% higher than with pure helium.