Potential of melt electrowritten scaffolds seeded with meniscus cells and mesenchymal stromal cells

Jasmijn V. Korpershoek; Mylène de Ruijter; Bastiaan F. Terhaard; Michella H. Hagmeijer; Daniël B.F. Saris; Miguel Castilho; Jos Malda; Lucienne A. Vonk

doi:10.3390/ijms222011200

Potential of melt electrowritten scaffolds seeded with meniscus cells and mesenchymal stromal cells

Jasmijn V. Korpershoek, Mylène de Ruijter, Bastiaan F. Terhaard, Michella H. Hagmeijer, Daniël B.F. Saris, Miguel Castilho, Jos Malda (Corresponding author), Lucienne A. Vonk (Corresponding author)

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Abstract

Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Polycaprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and-type for potential translation as a one-stage treatment.

Original language	English
Article number	11200
Number of pages	16
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	20
DOIs	https://doi.org/10.3390/ijms222011200
Publication status	Published - 18 Oct 2021

Bibliographical note

Funding Information:
Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research

Funding Information:
This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013).

Funding

Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013).

Keywords

Biofabrication
Clinical translation
Collagen meniscus implant®
Melt electrowriting
Meniscectomy18
Meniscus
Meniscus injury
Tissue-engineering
Proteoglycans/metabolism
Humans
Middle Aged
Cells, Cultured
Male
Elastic Modulus
Mesenchymal Stem Cells
Aged, 80 and over
Adult
Extracellular Matrix/chemistry
Female
Meniscus/cytology
Tissue Scaffolds/chemistry
Aged

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title = "Potential of melt electrowritten scaffolds seeded with meniscus cells and mesenchymal stromal cells",

abstract = "Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Polycaprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants{\textregistered} (CMI{\textregistered}). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI{\textregistered}, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young{\textquoteright}s modulus when compared to the CMI{\textregistered} scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and-type for potential translation as a one-stage treatment.",

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author = "Korpershoek, {Jasmijn V.} and {de Ruijter}, Myl{\`e}ne and Terhaard, {Bastiaan F.} and Hagmeijer, {Michella H.} and Saris, {Dani{\"e}l B.F.} and Miguel Castilho and Jos Malda and Vonk, {Lucienne A.}",

note = "Funding Information: Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research Funding Information: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013). ",

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AU - Hagmeijer, Michella H.

AU - Saris, Daniël B.F.

AU - Castilho, Miguel

AU - Malda, Jos

AU - Vonk, Lucienne A.

N1 - Funding Information: Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research Funding Information: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013).

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N2 - Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Polycaprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and-type for potential translation as a one-stage treatment.

AB - Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Polycaprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and-type for potential translation as a one-stage treatment.

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Potential of melt electrowritten scaffolds seeded with meniscus cells and mesenchymal stromal cells

Abstract

Bibliographical note

Funding

Keywords

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