PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

M. Srinivas, J. Tel, G. Schreibelt, F. Bonetto, L.-J. Cruz, Houshang Amiri, A. Heerschap, C.G. Figdor, I.J.M. de Vries

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.

MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.

RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.

CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.

Original languageEnglish
Pages (from-to)2339-48
Number of pages10
JournalNanomedicine
Volume10
Issue number15
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Cells, Cultured
  • Fluorine-19 Magnetic Resonance Imaging
  • Fluorocarbons
  • Humans
  • Lactic Acid
  • Lymphocyte Culture Test, Mixed
  • Microscopy, Electron, Scanning
  • Nanoparticles
  • Polyglycolic Acid
  • Journal Article
  • Research Support, Non-U.S. Gov't

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