PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

M. Srinivas; J. Tel; G. Schreibelt; F. Bonetto; L.-J. Cruz; Houshang Amiri; A. Heerschap; C.G. Figdor; I.J.M. de Vries

doi:10.2217/NNM.15.76

PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

M. Srinivas, J. Tel, G. Schreibelt, F. Bonetto, L.-J. Cruz, Houshang Amiri, A. Heerschap, C.G. Figdor, I.J.M. de Vries

Research output: Contribution to journal › Article › Academic › peer-review

38 Citations (Scopus)

Abstract

AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.

MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.

RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.

CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.

Original language	English
Pages (from-to)	2339-48
Number of pages	10
Journal	Nanomedicine
Volume	10
Issue number	15
DOIs	https://doi.org/10.2217/NNM.15.76
Publication status	Published - 2015
Externally published	Yes

Keywords

Cells, Cultured
Fluorine-19 Magnetic Resonance Imaging
Fluorocarbons
Humans
Lactic Acid
Lymphocyte Culture Test, Mixed
Microscopy, Electron, Scanning
Nanoparticles
Polyglycolic Acid
Journal Article
Research Support, Non-U.S. Gov't

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title = "PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI",

abstract = "AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.",

keywords = "Cells, Cultured, Fluorine-19 Magnetic Resonance Imaging, Fluorocarbons, Humans, Lactic Acid, Lymphocyte Culture Test, Mixed, Microscopy, Electron, Scanning, Nanoparticles, Polyglycolic Acid, Journal Article, Research Support, Non-U.S. Gov't",

author = "M. Srinivas and J. Tel and G. Schreibelt and F. Bonetto and L.-J. Cruz and Houshang Amiri and A. Heerschap and C.G. Figdor and {de Vries}, I.J.M.",

year = "2015",

doi = "10.2217/NNM.15.76",

language = "English",

volume = "10",

pages = "2339--48",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Taylor and Francis Ltd.",

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TY - JOUR

T1 - PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

AU - Srinivas, M.

AU - Tel, J.

AU - Schreibelt, G.

AU - Bonetto, F.

AU - Cruz, L.-J.

AU - Amiri, Houshang

AU - Heerschap, A.

AU - Figdor, C.G.

AU - de Vries, I.J.M.

PY - 2015

Y1 - 2015

N2 - AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.

AB - AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.

KW - Cells, Cultured

KW - Fluorine-19 Magnetic Resonance Imaging

KW - Fluorocarbons

KW - Humans

KW - Lactic Acid

KW - Lymphocyte Culture Test, Mixed

KW - Microscopy, Electron, Scanning

KW - Nanoparticles

KW - Polyglycolic Acid

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.2217/NNM.15.76

DO - 10.2217/NNM.15.76

M3 - Article

C2 - 26251876

SN - 1743-5889

VL - 10

SP - 2339

EP - 2348

JO - Nanomedicine

JF - Nanomedicine

IS - 15

ER -

PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

Abstract

Keywords

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