Abstract
AIM: In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.
MATERIALS & METHODS: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.
RESULTS: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.
CONCLUSION: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.
Original language | English |
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Pages (from-to) | 2339-48 |
Number of pages | 10 |
Journal | Nanomedicine |
Volume | 10 |
Issue number | 15 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Keywords
- Cells, Cultured
- Fluorine-19 Magnetic Resonance Imaging
- Fluorocarbons
- Humans
- Lactic Acid
- Lymphocyte Culture Test, Mixed
- Microscopy, Electron, Scanning
- Nanoparticles
- Polyglycolic Acid
- Journal Article
- Research Support, Non-U.S. Gov't